Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp

被引：6
作者：
Liu, F. [1 ]
Cao, Y. P. [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Plant Sci, Shanghai 200030, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 04期
关键词：
5-Enopyruvylshikimate-3-phosphate synthase; Pantoea sp; Clone; Escherichia coli; 5-ENOLPYRUVYLSHIKIMATE 3-PHOSPHATE SYNTHASE; GLYPHOSATE-RESISTANT CROPS; SHIKIMATE PATHWAY; EPSP SYNTHASE; SUBSTITUTION; FUTURE; GENES; SITE;
D O I：
10.4238/2015.December.29.33
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The shikimate pathway enzyme 5-enopyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. A novel aroA gene encoding an EPSPS from Pantoea sp was identified and subcloned into the pET-28a vector to construct the recombinant pET-AroA(Pantoea sp) plasmid. Amino acid sequence analysis indicated that AroA(Pantoea sp) is a class I AroA enzyme. When expressed in Escherichia coli, it conveyed high tolerance to glyphosate. AroA(Pantoea sp) may be used to generate transgenic glyphosate-tolerant plants.
引用
收藏
页码：19233 / 19241
页数：9
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