Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins

被引:0
|
作者
Ozel, Merve [1 ,2 ]
Guclu, Kenan [3 ]
Kilic, Eser [1 ]
Baskol, Mevlut [4 ]
Baskol, Gulden [1 ,2 ]
Helvaci, Nazli [1 ]
机构
[1] Erciyes Univ, Dept Biochem, Sch Med, Kayseri, Turkey
[2] Erciyes Univ, Betul Ziya Eren Genome & Stem Cell Ctr, Kayseri, Turkey
[3] Kirsehir Ahievran Univ Training & Res Hosp, Dept Biochem, Kirsehir, Turkey
[4] Erciyes Univ, Dept Gastroenterol, Sch Med, Kayseri, Turkey
来源
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI | 2021年 / 46卷 / 03期
关键词
hepatic fibrosis; hippo pathway; suberoylanilide hydroxamic acid; YAP/TAZ; HISTONE DEACETYLASES; LIVER FIBROSIS; YAP; CANCER;
D O I
10.1515/tjb-2019-0397
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Hepatic fibrosis is a complex and dynamic process similar to "wound healing" that results in the progressive accumulation of connective tissue. We aimed to investigate the epigenetic control of liver fibrosis and Hippo pathway in human hepatic stellate cell (HSC) line. We examined the effect of Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor on the LX2 cell line. Material and methods: 2.5 mu M SAHA was treated to LX2 cell line for 2 days. Cell proliferation and apoptosis measurement were performed by Muse Cell Analyzer. Yes-Associated Protein/Transcriptional Coactivator With Pdz-Binding Motif (YAP/TAZ) and alpha-smooth muscle actin (alpha-SMA) protein expression levels were measured by western blotting. Results: In our study, we observed that the SAHA treatment reduced cell viability and induced apoptosis of LX2 cells statistically. We found that SAHA treatment decreased alpha-SMA, YAP and TAZ proteins levels statistically. Conclusion: Decreased cell viability could be due to physiological, autophagical and also related to the apoptotical mechanisms. We thought that SAHA plays an important role in the creation of the fates of the LX2 cell line.
引用
收藏
页码:299 / 305
页数:7
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