Sex- and Tissue-Specific Functions of Drosophila Doublesex Transcription Factor Target Genes

被引：96

作者：

Clough, Emily ^{[1
]}

Jimenez, Erin ^{[2
]}

Kim, Yoo-Ah ^{[3
]}

Whitworth, Cale ^{[1
,2
]}

Neville, Megan C. ^{[4
]}

Hempel, Leonie U. ^{[1
]}

Pavlou, Hania J. ^{[4
]}

Chen, Zhen-Xia ^{[1
]}

Sturgill, David ^{[1
]}

Dale, Ryan K. ^{[1
]}

Smith, Harold E. ^{[1
]}

Przytycka, Teresa M. ^{[3
]}

Goodwin, Stephen F. ^{[4
]}

Van Doren, Mark ^{[2
]}

Oliver, Brian ^{[1
]}

机构：

[1] NIDDK, NIH, Bethesda, MD 20892 USA

[2] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

[3] NIH, Computat Biol Branch, NCBI, NLM, Bethesda, MD 20814 USA

[4] Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3PT, England

来源：

DEVELOPMENTAL CELL | 2014年 / 31卷 / 06期

基金：

英国惠康基金;

关键词：

DNA-BINDING; EXPRESSION ANALYSIS; JUVENILE-HORMONE; MELANOGASTER; EVOLUTION; DIFFERENTIATION; PROTEIN; VERTEBRATE; GERMLINE; DIMORPHISM;

D O I：

10.1016/j.devcel.2014.11.021

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Primary sex-determination "switches" evolve rapidly, but Doublesex (DSX)-related transcription factors (DMRTs) act downstream of these switches to control sexual development in most animal species. Drosophila dsx encodes female- and male-specific isoforms (DSXF and DSXM), but little is known about how dsx controls sexual development, whether DSXF and DSXM bind different targets, or how DSX proteins direct different outcomes in diverse tissues. We undertook genome-wide analyses to identify DSX targets using in vivo occupancy, binding site prediction, and evolutionary conservation. We find that DSXF and DSXM bind thousands of the same targets in multiple tissues in both sexes, yet these targets have sex- and tissue-specific functions. Interestingly, DSX targets show considerable overlap with targets identified for mouse DMRT1. DSX targets include transcription factors and signaling pathway components providing for direct and indirect regulation of sex- biased expression.

引用

页码：761 / 773

页数：13

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