Mapping of the continuous epitopes displayed on the Clostridium perfringens type D epsilon-toxin

被引：3

作者：

Alves, Guilherme Guerra ^{[1
]}

Machado-de-Avila, Ricardo Andrez ^{[2
,3
]}

Chavez-Olortegui, Carlos Delfin ^{[2
]}

Silveira Silvaa, Rodrigo Otavio ^{[1
]}

Faria Lobato, Francisco Carlos ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Escola Vet, Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Belo Horizonte, MG, Brazil

[3] Univ Extremo Sul Catarinense UNESC, Criciuma, SC USA

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2017年 / 48卷 / 03期

关键词：

Epsilon; Toxin; Enterotoxemia; Epitope; Mappinga; PORE-FORMING TOXIN; NEUTRALIZING ANTIBODIES; PROTECTIVE ANTIGEN; AMINO-ACIDS; ALPHA-TOXIN; IMMUNIZATION; BINDING; IDENTIFICATION; MEMBRANE; PEPTIDE;

D O I：

10.1016/j.bjm.2016.10.023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia inruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vaccines. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.

引用

页码：570 / 575

页数：6

共 26 条

[11] Identification of Amino Acids Important for Binding of Clostridium perfringens Epsilon Toxin to Host Cells and to HAVCR1 [J].

Ivie, Susan E. ;

McClain, Mark S. .

BIOCHEMISTRY, 2012, 51 (38) :7588-7595

[12] Identification of the channel-forming domain of Clostridium perfringens Epsilon-toxin (ETX) [J].

Knapp, Oliver ;

Maier, Elke ;

Benz, Roland ;

Geny, Blandine ;

Popoff, Michel R. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2009, 1788 (12) :2584-2593

[13]

Kukic P, 2010, FUTURE MED CHEM, V2, P647, DOI [10.4155/fmc.10.6, 10.4155/FMC.10.6]

[14] Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display [J].

Levy, R. ;

Forsyth, C. M. ;

LaPorte, S. L. ;

Geren, I. N. ;

Smith, L. A. ;

Marks, J. D. .

JOURNAL OF MOLECULAR BIOLOGY, 2007, 365 (01) :196-210

[15] Functional analysis of neutralizing antibodies against Clostridium perfringens epsilon-toxin [J].

McClain, Mark S. ;

Cover, Timothy L. .

INFECTION AND IMMUNITY, 2007, 75 (04) :1785-1793

[16]

MERRIFIELD RB, 1969, ADV ENZYMOL RAMB, V32, P221

[17] Cleavage of a C-terminal peptide is essential for heptamerization of Clostridium perfringens ε-toxin in the synaptosomal membrane [J].

Miyata, S ;

Matsushita, O ;

Minami, J ;

Katayama, S ;

Shimamoto, S ;

Okabe, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (17) :13778-13783

[18] Production of a non-toxic site-directed mutant of Clostridium perfringens ε-toxin which induces protective immunity in mice [J].

Oyston, PCF ;

Payne, DW ;

Havard, HL ;

Williamson, ED ;

Titball, RW .

MICROBIOLOGY-SGM, 1998, 144 :333-341

[19] Production and purification of epsilon prototoxin produced by Clostridium perfringens type D [J].

Parreiras, PM ;

Lobato, FCF ;

Heneine, CFLGD ;

Assis, RA ;

Balsamao, GM ;

Nascimento, RAP .

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, 2002, 54 (03) :328-330

[20] Epsilon toxin: a fascinating pore-forming toxin [J].

Popoff, Michel R. .

FEBS JOURNAL, 2011, 278 (23) :4602-4615

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