Cytotoxicity Studies of the Chemical Constituents from Marine Algae Chara baltica

For the first time, 89 chemical constituents were identified by gas chromatography-mass spectrometry analysis of ethanol extract of Clathria baltica. The chemical examination of ethanol extract of Clathria baltica yielded three known metabolites, 1, 2 and 3, for first time from the marine algae Clathria baltica. All three metabolites and the ethanol extract of Clathria baltica were screened against cancer cell lines, MCF-7, DLD-1, HeLa, FADU, A549, and SKOV3, and one normal human cell line using sulforhodamine B assay and doxorubicin as the standard. Among all isolates, compound 3 showed significant growth inhibition of MCF-7, DLD-1 and FADU with IC50 values of 26.5, 15.5 and 16.5 mu g/ml, respectively. Whereas, all the metabolites and the extract exerted lower growth inhibitory effect on the normal human cell line tested. This is the first in vitro gas chromatography-mass spectrometry analysis, as well as, cytotoxicity report on marine algae Clathria baltica.