Configurations used in the design of screen-printed enzymatic biosensors.: A review

被引：252
作者：
Albareda-Sirvent, M [1 ]
Merkoçi, A [1 ]
Alegret, S [1 ]
机构：
[1] Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra 08193, Catalonia, Spain
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2000年 / 69卷 / 1-2期
关键词：
enzyme immobilisation; thick-film biosensors; screen-printed biosensors; screen-printing ink; screen-printing paste;
D O I：
10.1016/S0925-4005(00)00536-0
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Different thick-film biosensor configurations designed during the last decade are revised. These planar configurations are classified in three groups: (a) multiple-layer deposition (biological deposition by hand or electrochemically), (b) screen-printing of composite inks or pastes using two or more steps (biological deposition by screen-printing), (c) one-step deposition layer or biocomposite strategy. Different enzyme immobilisation procedures corresponding to these configurations are also revised. These procedures consist mainly of enzyme adsorption onto transducer surface, biological immobilisation by cross-linking in a glutaraldehyde layer, and physical, electro-or UV-polymerisation entrapment. Immobilisation into the bulk of different carbon-based inks or pastes is also presented. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码：153 / 163
页数：11
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