Evaluation of doubled haploid culture conditions and regeneration of an indica rice hybrid

被引：4

作者：

Bhattacharya, Anjanabha ^{[1
]}

Mikkilineni, Venugopal ^{[1
]}

Verma, Laxmikant ^{[1
]}

Palan, Bhavesh ^{[1
]}

Mali, Kishor ^{[1
]}

Char, Bharat ^{[1
]}

机构：

[1] Maharashtra Hybrid Seeds Co Ltd, Mahyco Res Ctr, Jalna 431203, Maharashtra, India

来源：

INDIAN JOURNAL OF GENETICS AND PLANT BREEDING | 2014年 / 74卷 / 03期

关键词：

Rice; doubled haploid (DH); anther culture; hybrid; callus induction; plant regeneration; genetic analysis; ANTHER CULTURE;

D O I：

10.5958/0975-6906.2014.00858.X

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A study was undertaken to develop an effective protocol to generate doubled haploid plants from a proprietary Mahyco hybrid MRP5401. A total of 20,300 anthers were plated on four different DH media (basal N6 + phytohormones) and obtained a callus induction success rate of 1.52%, of which 0.48% developed into green plants. A total of 232 lines were obtained of which, 98 lines showed homozygosity in DH2-3 generations. Well established DH lines from MRP5401 in DH3 generation had early flowering and yield gain.

引用

页码：384 / 386

页数：3

共 9 条

[1]

Ambarwati A. D., 2009, J AGROBIOGEN, V5, P71

[2]

Datta SK, 2005, CURR SCI INDIA, V89, P1870

[3] Anther culture for haploid and doubled haploid production [J].

Germana, Maria Antonietta .

PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 104 (03) :283-300

[4] Anther culture performance in selected high yielding indica (of Sri Lanka) and japonica rice varieties and their inter sub-specific hybrids [J].

Herath, H. M. I. ;

Bandara, D. C. .

JOURNAL OF THE NATIONAL SCIENCE FOUNDATION OF SRI LANKA, 2011, 39 (02) :149-154

[5]

Herath H. M. I., 2009, Ceylon Journal of Science, Biological Sciences, V38, P11, DOI 10.4038/cjsbs.v38i1.1322

[6] Promotion of androgenesis in rice anther cultures by substitution of sucrose with maltose and mannitol [J].

Pande, H ;

Bhojwani, SS .

BIOLOGIA PLANTARUM, 1999, 42 (01) :125-128

[7]

Ramakrishnan S. H., 2005, Asian Journal of Plant Sciences, V4, P600

[8]

Sasmita P, 2010, NUSANT BIOSCI, V2, P67

[9]

SATHISH P, 1995, PLANT CELL REP, V14, P432, DOI 10.1007/BF00234049

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