Stable Small Quantum Dots for Synaptic Receptor Tracking on Live Neurons

被引：59

作者：

Cai, En ^{[1
,2
]}

Ge, Pinghua ^{[1
,2
]}

Lee, Sang Hak ^{[1
,2
]}

Jeyifous, Okunola ^{[1
,2
,3
]}

Wang, Yong ^{[1
,2
]}

Liu, Yanxin ^{[1
,2
,4
]}

Wilson, Katie M. ^{[5
,6
]}

Lim, Sung Jun ^{[7
]}

Baird, Michelle A. ^{[8
,9
]}

Stone, John E. ^{[4
]}

Lee, Kwan Young ^{[10
]}

Davidson, Michael W. ^{[8
,9
]}

Chung, Hee Jung ^{[10
]}

Schulten, Klaus ^{[1
,2
,4
,11
]}

Smith, Andrew M. ^{[7
]}

Green, William N. ^{[3
,12
]}

Selvin, Paul R. ^{[1
,2
,11
,12
]}

机构：

[1] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

[2] Univ Illinois, Ctr Phys Living Cells, Urbana, IL 61801 USA

[3] Univ Chicago, Dept Neurobiol, Chicago, IL 60637 USA

[4] Univ Illinois, Beckman Inst, Urbana, IL 61801 USA

[5] Univ Liverpool, Dept Struct & Chem Biol, Liverpool L69 3BX, Merseyside, England

[6] Univ Liverpool, Inst Integrat Biol, Liverpool L69 3BX, Merseyside, England

[7] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA

[8] Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32306 USA

[9] Florida State Univ, Dept Biol Sci, Tallahassee, FL 32306 USA

[10] Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA

[11] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA

[12] Marine Biol Lab, Woods Hole, MA 02543 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 46期

基金：

美国国家科学基金会;

关键词：

AMPA receptors; fluorescent probes; quantum dots; single-molecule studies; LIVING CELLS; BIOTIN LIGASE; PROTEINS; NANOPARTICLES; MONOVALENT; DYNAMICS; MONOFUNCTIONALIZATION; TRAFFICKING; TRANSPORT; SYNAPSES;

D O I：

10.1002/anie.201405735

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We developed a coating method to produce functionalized small quantum dots (sQDs), about 9nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.

引用

页码：12484 / 12488

页数：5

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