Long-term monitoring of SARS-CoV-2 RNA in sewage samples from specific public places and STPs to track COVID-19 spread and identify potential hotspots

被引：18

作者：

de Araujo, Juliana Calabria ^{[1
]}

Mota, Vera Taina ^{[1
]}

Teodoro, Amanda ^{[1
]}

Leal, Cintia ^{[1
]}

Leroy, Deborah ^{[1
]}

Madeira, Camila ^{[1
]}

Machado, Elayne C. ^{[1
]}

Dias, Marcela F. ^{[1
]}

Souza, Cassia C. ^{[1
]}

Coelho, Gabriela ^{[1
]}

Bressani, Thiago ^{[1
]}

Morandi, Thiago ^{[1
]}

Freitas, Gabriel Tadeu O. ^{[1
]}

Duarte, Alyne ^{[1
]}

Perdigao, Carlos ^{[2
]}

Troger, Flavio ^{[2
]}

Ayrimoraes, Sergio ^{[2
]}

de Melo, Marilia Carvalho ^{[3
]}

Laguardia, Filipe ^{[4
]}

Reis, Marcus Tulius P. ^{[5
]}

Mota, Cesar ^{[1
]}

Chernicharo, Carlos A. L. ^{[1
]}

机构：

[1] Fed Univ Minas Gerais UFMG, Dept Sanit & Environm Engn DESA, Belo Horizonte, MG, Brazil

[2] Natl Agcy Water & Sanitat ANA, Belo Horizonte, MG, Brazil

[3] Secretary State Environm & Sustainable Dev SEMAD, Belo Horizonte, MG, Brazil

[4] Minas Gerais State Hlth Author SES, Belo Horizonte, MG, Brazil

[5] Sanitat Co Minas Gerais COPASA, Belo Horizonte, MG, Brazil

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2022年 / 838卷

关键词：

SARS-CoV-2; Waterwater-based epidemiology; Sentinel sewage surveillance; Public places; International airport; WATER REUSE;

D O I：

10.1016/j.scitotenv.2022.155959

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Coronavirus pandemic started in March 2020 and since then has caused millions of deaths worldwide. Wastewater-based epidemiology (WBE) can be used as an epidemiological surveillance tool to track SARS-CoV-2 dissemination and provide warning of COVID-19 outbreaks. Considering that there are public places that could be potential hotspots of infected people that may reflect the local epidemiological situation, the presence of SARS-CoV-2 RNA was analyzed by RT-qPCR for approximately 16 months in sewage samples from five public places located in the metropolitan area of Belo Horizonte, MG, Brazil: the sewage treatment plant of Confins International Airport (AIR), the main interstate bus terminal (BUS), an upscale shopping centre (SHC1), a popular shopping centre (SHC2) and a university institute (UNI). The results were compared to those of the influent sewage of the two main sewage treatment plants of Belo Horizonte (STP1 and STP2). Viral monitoring in the STPs proved to be an useful regional surveillance tool, reflecting the trends of COVID-19 cases. However, the viral concentrations in the samples from the selected public places were generally much lower than those of the municipal STPs, which may be due to the behaviour of the non-infected or asymptomatic people, who are likely to visit these places relatively more than the symptomatic infected ones. Among these places, the AIR samples presented the highest viral concentrations and concentration peaks were observed previously to local outbreaks. Therefore, airport sewage monitoring can provide an indication of the regional epidemiological situation. For the other places, particularly the UNI, the results suggested a greater potential to detect the infection and trace cases especially among employees and regular attendees. Taken together, the results indicate that for a regular and permanent sentinel sewage surveillance the sewage from STPs, AIR and UNI could be monitored.

引用

页数：11

共 59 条

[41] Wastewater-based epidemiology as a useful tool to track SARS-CoV-2 and support public health policies at municipal level in Brazil [J].

Prado, Tatiana ;

Fumian, Tulio Machado ;

Mannarino, Camille Ferreira ;

Resende, Paola Cristina ;

Motta, Fernando Couto ;

Fontes Eppinghaus, Ana Lucia ;

Chagas do Vale, Vitor Hugo ;

Soares Braz, Ricardo Marinho ;

Ribeiro de Andrade, Juliana da Silva ;

Maranhao, Adriana Goncalves ;

Miagostovich, Marize Pereira .

WATER RESEARCH, 2021, 191

[42] SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area [J].

Randazzo, Walter ;

Truchado, Pilar ;

Cuevas-Ferrando, Enric ;

Simon, Pedro ;

Allende, Ana ;

Sanchez, Gloria .

WATER RESEARCH, 2020, 181

[43] Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers [J].

Rimoldi, Sara Giordana ;

Stefani, Fabrizio ;

Gigantiello, Anna ;

Polesello, Stefano ;

Comandatore, Francesco ;

Mileto, Davide ;

Maresca, Mafalda ;

Longobardi, Concetta ;

Mancon, Alessandro ;

Romeri, Francesca ;

Pagani, Cristina ;

Cappelli, Francesca ;

Roscioli, Claudio ;

Moja, Lorenzo ;

Gismondo, Maria Rita ;

Salerno, Franco .

SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 744

[44]

RIVM-National Institute for Public Health and the Environment, COR MON SEW RES

[45] Targeted wastewater surveillance of SARS-CoV-2 on a university campus for COVID-19 outbreak detection and mitigation [J].

Scott, Laura C. ;

Aubee, Alexandra ;

Babahaji, Layla ;

Vigil, Katie ;

Tims, Scott ;

Aw, Tiong Gim .

ENVIRONMENTAL RESEARCH, 2021, 200

[46] First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA [J].

Sherchan, Samendra P. ;

Shahin, Shalina ;

Ward, Lauren M. ;

Tandukar, Sarmila ;

Aw, Tiong G. ;

Schmitz, Bradley ;

Ahmed, Warish ;

Kitajima, Masaaki .

SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 743

[47] A case study of enteric virus removal and insights into the associated risk of water reuse for two wastewater treatment pond systems in Bolivia [J].

Symonds, E. M. ;

Verbyla, M. E. ;

Lukasik, J. O. ;

Kafle, R. C. ;

Breitbart, M. ;

Mihelcic, J. R. .

WATER RESEARCH, 2014, 65 :257-270

[48] Microbial indicators of faecal contamination in water: A current perspective [J].

Tallon, P ;

Magajna, B ;

Lofranco, C ;

Leung, KT .

WATER AIR AND SOIL POLLUTION, 2005, 166 (1-4) :139-166

[49] Detection of SARS-CoV-2 RNA in wastewater, river water, and hospital wastewater of Nepal [J].

Tandukar, Sarmila ;

Sthapit, Niva ;

Thakali, Ocean ;

Malla, Bikash ;

Sherchan, Samendra P. ;

Shakya, Bijay Man ;

Shrestha, Laxman P. ;

Sherchand, Jeevan B. ;

Joshi, Dev Raj ;

Lama, Bhupendra ;

Haramoto, Eiji .

SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 824

[50] Managing Microbial Risks from Indirect Wastewater Reuse for Irrigation in Urbanizing Watersheds [J].

Verbyla, Matthew E. ;

Symonds, Erin M. ;

Kafle, Ram C. ;

Cairns, Maryann R. ;

Iriarte, Mercedes ;

Mercado Guzman, Alvaro ;

Coronado, Olver ;

Breitbart, Mya ;

Ledo, Carmen ;

Mihelcic, James R. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2016, 50 (13) :6803-6813

← 1 2 3 4 5 6 →