Long-term monitoring of SARS-CoV-2 RNA in sewage samples from specific public places and STPs to track COVID-19 spread and identify potential hotspots

被引：17

作者：

de Araujo, Juliana Calabria ^{[1
]}

Mota, Vera Taina ^{[1
]}

Teodoro, Amanda ^{[1
]}

Leal, Cintia ^{[1
]}

Leroy, Deborah ^{[1
]}

Madeira, Camila ^{[1
]}

Machado, Elayne C. ^{[1
]}

Dias, Marcela F. ^{[1
]}

Souza, Cassia C. ^{[1
]}

Coelho, Gabriela ^{[1
]}

Bressani, Thiago ^{[1
]}

Morandi, Thiago ^{[1
]}

Freitas, Gabriel Tadeu O. ^{[1
]}

Duarte, Alyne ^{[1
]}

Perdigao, Carlos ^{[2
]}

Troger, Flavio ^{[2
]}

Ayrimoraes, Sergio ^{[2
]}

de Melo, Marilia Carvalho ^{[3
]}

Laguardia, Filipe ^{[4
]}

Reis, Marcus Tulius P. ^{[5
]}

Mota, Cesar ^{[1
]}

Chernicharo, Carlos A. L. ^{[1
]}

机构：

[1] Fed Univ Minas Gerais UFMG, Dept Sanit & Environm Engn DESA, Belo Horizonte, MG, Brazil

[2] Natl Agcy Water & Sanitat ANA, Belo Horizonte, MG, Brazil

[3] Secretary State Environm & Sustainable Dev SEMAD, Belo Horizonte, MG, Brazil

[4] Minas Gerais State Hlth Author SES, Belo Horizonte, MG, Brazil

[5] Sanitat Co Minas Gerais COPASA, Belo Horizonte, MG, Brazil

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2022年 / 838卷

关键词：

SARS-CoV-2; Waterwater-based epidemiology; Sentinel sewage surveillance; Public places; International airport; WATER REUSE;

D O I：

10.1016/j.scitotenv.2022.155959

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Coronavirus pandemic started in March 2020 and since then has caused millions of deaths worldwide. Wastewater-based epidemiology (WBE) can be used as an epidemiological surveillance tool to track SARS-CoV-2 dissemination and provide warning of COVID-19 outbreaks. Considering that there are public places that could be potential hotspots of infected people that may reflect the local epidemiological situation, the presence of SARS-CoV-2 RNA was analyzed by RT-qPCR for approximately 16 months in sewage samples from five public places located in the metropolitan area of Belo Horizonte, MG, Brazil: the sewage treatment plant of Confins International Airport (AIR), the main interstate bus terminal (BUS), an upscale shopping centre (SHC1), a popular shopping centre (SHC2) and a university institute (UNI). The results were compared to those of the influent sewage of the two main sewage treatment plants of Belo Horizonte (STP1 and STP2). Viral monitoring in the STPs proved to be an useful regional surveillance tool, reflecting the trends of COVID-19 cases. However, the viral concentrations in the samples from the selected public places were generally much lower than those of the municipal STPs, which may be due to the behaviour of the non-infected or asymptomatic people, who are likely to visit these places relatively more than the symptomatic infected ones. Among these places, the AIR samples presented the highest viral concentrations and concentration peaks were observed previously to local outbreaks. Therefore, airport sewage monitoring can provide an indication of the regional epidemiological situation. For the other places, particularly the UNI, the results suggested a greater potential to detect the infection and trace cases especially among employees and regular attendees. Taken together, the results indicate that for a regular and permanent sentinel sewage surveillance the sewage from STPs, AIR and UNI could be monitored.

引用

页数：11

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