A microfluidic device for capture of single cells and impedance measurement

被引：0

作者：

Wang, Min-Haw ^{[1
]}

Kao, Min-Feng ^{[1
]}

Liu, Haw-Juin ^{[1
]}

Kan, Wai-Hong ^{[1
]}

Hsu, Yi-Chu ^{[2
]}

Jang, Ling-Sheng ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Dept Elect Engn, Tainan 70101, Taiwan

[2] Southern Taiwan Univ Technol, Dept Engn Mech, Tainan, Taiwan

来源：

2007 2ND IEEE INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS, VOLS 1-3 | 2007年

关键词：

single cell; lab-on-a-chip; MEMS; impedance sensor;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A microfluidic device for capture of single cells and impedance measurement is presented. The device consists of a PDMS channel with three micro pillars and a glass substrate with electrodes. The experiments demonstrated that the HeLa cell (human cervical epithelioid carcinoma) was successfully captured by the micro pillars and its impedance was measured by impedance spectroscopy. The range of operation voltage is from 0.1 V to 1.5 V and the scan frequency is from 1 kHz to 100 kHz. According to experimental results, the HeLa cell is capacitive and its electrical model can be simplified to the parallel connection with one resistor and one capacitor. This developed technique for cell impedance analysis possesses advantages of physical capture, low cost, and easy of fabrication and measurement.

引用

页码：445 / +

页数：2

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