Inhibition of inducible nitric oxide synthesis by oxidized lipoprotein(a) in a murine macrophage cell line

被引：17

作者：

Moeslinger, T ^{[1
]}

Friedl, R ^{[1
]}

Volf, I ^{[1
]}

Brunner, M ^{[1
]}

Koller, E ^{[1
]}

Spieckermann, PG ^{[1
]}

机构：

[1] Inst Physiol, A-1090 Vienna, Austria

来源：

FEBS LETTERS | 2000年 / 478卷 / 1-2期

关键词：

nitric oxide; lipoprotein(a); mouse macrophage; atherosclerosis; inducible nitric oxide synthase;

D O I：

10.1016/S0014-5793(00)01825-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Increased plasma levels of human lipoprotein(a) (Lp(a)) are highly correlated with the development of atherosclerotic lesions. During our study, we investigated the effects of native and hypochlorite oxidized lipoprotein(a) (ox-Lp(a)) on nitric oxide production by the inducible nitric oxide synthase (iNOS) in lipopolysaccharide/interferon stimulated mouse macrophages (J774A.1). Ox-Lp(a) (0-2 mu g/ml) induces a dose dependent inhibition of inducible nitric oside synthesis. iNOS protein expression showed a dose dependent reduction as revealed by immunoblotting when cells were incubated with increasing amounts of ox-Lp(a). Ox-Lp(a) decreases iNOS mRNA synthesis as shown by reverse transcription-polymerase chain reaction. Os-Lp(a) induced iNOS inhibition might contribute to the development of atherosclerotic lesions by reducing the antiatherogenic effects of nitric oxide. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：95 / 99

页数：5

共 52 条

[1]

ARNHOLD J, 1991, BIOMED BIOCHIM ACTA, V50, P967

[2] Cyclooxygenase-2 is widely expressed in atherosclerotic lesions affecting native and transplanted human coronary arteries and colocalizes with inducible nitric oxide synthase and nitrotyrosine particularly in macrophages [J].