Modified RNAs in CRISPR/Cas9: An Old Trick Works Again

被引：17

作者：

Latorre, Alfonso ^{[1
]}

Latorre, Ana ^{[1
]}

Somoza, Alvaro ^{[1
]}

机构：

[1] Ctr Nacl Biotecnol CSIC, Unidad Asociada, IMDEA Nanociencia & Nanobiotecnol IMDEA Nanocienc, Faraday 9, Madrid 28049, Spain

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 11期

关键词：

CRISPR; Cas; gene editing; nucleotide analogues; nucleotides; RNA; GENOME; CRISPR-CAS9; CHALLENGES; DELIVERY; SYSTEM; CELLS; CAS;

D O I：

10.1002/anie.201512002

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Old tricks, new dog: CRISPR/Cas9 is a powerful tool for gene editing that requires an endonuclease (Cas9) and RNA strands. It has been shown that chemical modification of the RNA structures, an approach that has been used to improve the efficiency of RNA interference, can also be applied to enhance the activity of CRISPR/Cas9 and reduce its off-target effects. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：3548 / 3550

页数：3

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