Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions

被引：33

作者：

Kutikhin, Anton G. ^{[1
]}

Velikanova, Elena A. ^{[1
]}

Mukhamadiyarov, Rinat A. ^{[1
]}

Glushkova, Tatiana V. ^{[1
]}

Borisov, Vadim V. ^{[1
]}

Matveeva, Vera G. ^{[1
]}

Antonova, Larisa V. ^{[1
]}

Filip'ev, Dmitriy E. ^{[1
]}

Golovkin, Alexey S. ^{[1
]}

Shishkova, Daria K. ^{[1
]}

Burago, Andrey Yu. ^{[1
]}

Frolov, Alexey V. ^{[1
]}

Dolgov, Viktor Yu. ^{[1
]}

Efimova, Olga S. ^{[2
]}

Popova, Anna N. ^{[2
]}

Malysheva, Valentina Yu. ^{[2
]}

Vladimirov, Alexandr A. ^{[2
]}

Sozinov, Sergey A. ^{[2
]}

Ismagilov, Zinfer R. ^{[2
]}

Russakov, Dmitriy M. ^{[3
]}

Lomzov, Alexander A. ^{[4
,5
]}

Pyshnyi, Dmitriy V. ^{[4
,5
]}

Gutakovsky, Anton K. ^{[6
]}

Zhivodkov, Yuriy A. ^{[6
]}

Demidov, Evgeniy A. ^{[7
]}

Peltek, Sergey E. ^{[5
,7
]}

Dolganyuk, Viatcheslav F. ^{[8
]}

Babich, Olga O. ^{[8
]}

Grigoriev, Evgeniy V. ^{[1
,9
]}

Brusina, Elena B. ^{[1
,9
]}

Barbarash, Olga L. ^{[1
,9
]}

Yuzhalin, Arseniy E. ^{[1
,10
,11
]}

机构：

[1] Res Inst Complex Issues Cardiovasc Dis, Sosnovy Blvd 6, Kemerovo 650002, Russia

[2] Russian Acad Sci, Inst Coal Chem & Mat Sci, Siberian Branch, Sovietsky Ave 18, Kemerovo 650000, Russia

[3] Kemerovo State Univ, Krasnaya St 6, Kemerovo 650043, Russia

[4] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Lavrentiev Ave 8, Novosibirsk 630090, Russia

[5] Novosibirsk State Univ, Pirogova St 2, Novosibirsk 630090, Russia

[6] Russian Acad Sci, Rzhanov Inst Semicond Phys, Siberian Branch, Prospekt Lavrentieva 13, Novosibirsk 630090, Russia

[7] Russian Acad Sci, Inst Cytol & Genet, Siberian Branch, Prospekt Lavrentieva 10, Novosibirsk 630090, Russia

[8] Kemerovo Inst Food Sci & Technol, Biotechnol Res Inst, Stroiteley Blvd 47, Kemerovo 650056, Russia

[9] Kemerovo State Med Acad, Voroshilova St 22a, Kemerovo 650029, Russia

[10] Univ Oxford, Canc Res UK, Dept Oncol, Old Rd Campus,Res Bldg,Roosevelt Dr, Oxford OX3 7DQ, England

[11] Univ Oxford, Med Res Council, Oxford Inst Radiat Oncol, Old Rd Campus Res Bldg,Roosevelt Dr, Oxford OX3 7DQ, England

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

SERUM PHOSPHORUS LEVELS; CORONARY-HEART-DISEASE; ATHEROSCLEROSIS RISK; FETUIN-A; CARDIOVASCULAR-DISEASE; ANNULAR CALCIFICATION; VALVE CALCIFICATION; ELECTRON-MICROSCOPY; MAMMARY ARTERY; NANOBACTERIA;

D O I：

10.1038/srep27255

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Calcium phosphate bions (CPB) are biomimetic mineralo-organic nanoparticles which represent a physiological mechanism regulating the function, transport and disposal of calcium and phosphorus in the human body. We hypothesised that CPB may be pathogenic entities and even a cause of cardiovascular calcification. Here we revealed that CPB isolated from calcified atherosclerotic plaques and artificially synthesised CPB are morphologically and chemically indistinguishable entities. Their formation is accelerated along with the increase in calcium salts-phosphates/serum concentration ratio. Experiments in vitro and in vivo showed that pathogenic effects of CPB are defined by apoptosis-mediated endothelial toxicity but not by direct tissue calcification or functional changes in anti-calcification proteins. Since the factors underlying the formation of CPB and their pathogenic mechanism closely resemble those responsible for atherosclerosis development, further research in this direction may help us to uncover triggers of this disease.

引用

页数：16

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