Concentration and prevalence of Escherichia coli O157 in sheep faeces at pasture in Scotland

被引:40
作者
Ogden, ID [1 ]
MacRae, M
Strachan, NJC
机构
[1] Univ Aberdeen, Dept Med Microbiol, Appl Food Microbiol Grp, Aberdeen AB25 2ZD, Scotland
[2] Univ Aberdeen, Sch Biol Sci, Aberdeen AB25 2ZD, Scotland
关键词
concentration and prevalence; Escherichia coli O157; immunomagnetic separation; sheep;
D O I
10.1111/j.1365-2672.2004.02493.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To study the presence, numbers and virulence profiles of Escherichia coli O157 in sheep faeces and validate the microbiological methods used to attain these data. Methods and Results: Flock level prevalence was found to be 40% ( six from 15) and 6(.)5% of faecal samples tested were found to be positive. Two farms gave samples defined as high shedding (>10(4) CFU g(-1)), one of which comprised 91% positive samples with 13/33 at the high shedding level. Conclusions: These data confirmed that sheep are an important reservoir of E. coli O157. Significance and Impact of the Study: Sheep play a significant role in the maintenance and dispersal of E. coli O157 in the farming environment and are an important source of human infection.
引用
收藏
页码:646 / 651
页数:6
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