Zinc protects against arsenic-induced apoptosis in a neuronal cell line, measured by DEVD-caspase activity

被引：29

作者：

Milton, AG ^{[1
]}

Zalewski, PD ^{[1
]}

Ratnaike, RN ^{[1
]}

机构：

[1] Queen Elizabeth Hosp, Dept Med, Woodville, SA 5011, Australia

来源：

BIOMETALS | 2004年 / 17卷 / 06期

关键词：

arsenic trioxide; 2.3D mouse neuroepithelial cells; DEVD-caspase; zinc sulfate;

D O I：

10.1007/s10534-004-1210-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acute and chronic arsenic exposure results in toxicity in humans and causes many neurological and other manifestations. For the first time the present study reports that zinc decreases arsenic-induced apoptosis and also confirms a single report of apoptosis induced by arsenic in a neuronal cell line. Apoptosis measured by DEVD-caspase activity peaked between 10 muM and 20 muM of arsenic trioxide. Higher concentrations of arsenic up to 40 muM caused increasing cell death with diminishing DEVD-caspase activity. The beneficial effect of zinc was proportional to its concentration with a significant decrease in arsenic-induced DEVD-caspase activity at 50 muM and 75 muM zinc (P < 0.05). This finding may be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem especially relevant to millions of people on the Indian subcontinent.

引用

页码：707 / 713

页数：7

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