Genome-wide Mapping of the Nucleosome Landscape by Micrococcal Nuclease and Chemical Mapping

被引:23
|
作者
Voong, Lilien N. [1 ]
Xi, Liqun [2 ]
Wang, Ji-Ping [2 ]
Wang, Xiaozhong [1 ]
机构
[1] Northwestern Univ, Dept Mol Biosci, Evanston, IL 60208 USA
[2] Northwestern Univ, Dept Stat, Evanston, IL 60208 USA
关键词
RNA-POLYMERASE-II; PIONEER TRANSCRIPTION FACTORS; BASE-PAIR RESOLUTION; EMBRYONIC STEM-CELLS; DNA-BINDING PROTEINS; CHROMATIN-STRUCTURE; GENE-REGULATION; IN-VIVO; SACCHAROMYCES-CEREVISIAE; REGULATORY REGIONS;
D O I
10.1016/j.tig.2017.05.007
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Nucleosomes regulate the transcription output of the genome by occluding the underlying DNA sequences from DNA-binding proteins that must act on it. Knowledge of the precise locations of nucleosomes in the genome is thus essential towards understanding how transcription is regulated. Current nucleosome-mapping strategies involve digesting chromatin with nucleases or chemical cleavage followed by high-throughput sequencing. In this review, we compare the traditional micrococcal nuclease (MNase)-based approach with a chemical cleavage strategy, with discussion on the important insights each has uncovered about the role of nucleosomes in shaping transcriptional processes.
引用
收藏
页码:495 / 507
页数:13
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