Neospora caninum antibodies detected in Midwestern white-tailed deer (Odocoileus virginianus) by Western blot and ELISA

被引：26
作者：
Anderson, Todd
DeJardin, Amanda
Howe, Daniel K.
Dubey, J. P.
Michalski, Michelle L. [1 ]
机构：
[1] Univ Wisconsin, Dept Biol & Microbiol, Oshkosh, WI 54901 USA
[2] Univ Kentucky, Dept Vet Sci, Lexington, KY 40546 USA
[3] USDA, ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA
来源：
VETERINARY PARASITOLOGY | 2007年 / 145卷 / 1-2期
关键词：
Neospora caninum; white-tailed deer (Odocoileus virginianus); seropositivity; western blot; ELISA; Missouri; Wisconsin;
D O I：
10.1016/j.vetpar.2006.11.012
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
White-tailed deer (Odocoileus virginianus) serve to maintain the Neospora caninum life cycle in the wild. Sera from white-tailed deer from south central Wisconsin and southeastern Missouri, USA were tested for antibodies to N. caninum by Western blot analyses and two indirect ELISAs. Seroreactivity against N. caninum surface antigens was observed in 30 of 147 (20%) of WI deer and I I of 23 (48%) of MO deer using Western blot analysis. Compared to Western blot, the two indirect ELISAs were found to be uninformative due to degradation of the field-collected samples. The results indicate the existence of N. caninum antibodies in MO and WI deer, and that Western blot is superior to ELISA for serologic testing when using degraded blood samples collected from deer carcasses. (c) 2006 Elsevier B.V. All rights reserved.
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收藏
页码：152 / 155
页数：4
相关论文
共 19 条
[1]   IDENTIFICATION AND CHARACTERIZATION OF NEOSPORA-CANINUM TACHYZOITE ANTIGENS USEFUL FOR DIAGNOSIS OF NEOSPOROSIS [J].
BJERKAS, I ;
JENKINS, MC ;
DUBEY, JP .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1994, 1 (02) :214-221
[2]   DETECTION OF SERUM ANTIBODY-RESPONSES IN CATTLE WITH NATURAL OR EXPERIMENTAL NEOSPORA INFECTIONS [J].
CONRAD, PA ;
SVERLOW, K ;
ANDERSON, M ;
ROWE, J ;
BONDURANT, R ;
TUTER, G ;
BREITMEYER, R ;
PALMER, C ;
THURMOND, M ;
ARDANS, A ;
DUBEY, JP ;
DUHAMEL, G ;
BARR, B .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1993, 5 (04) :572-578
[3]   Diagnosis of bovine neosporosis [J].
Dubey, J. P. ;
Schares, G. .
VETERINARY PARASITOLOGY, 2006, 140 (1-2) :1-34
[4]  
Dubey J.P., 2003, J PARASITOL S, V89, pS42
[5]   High prevalence of antibodies to Neospora caninum in white-tailed deer (Odocoileus virginianus) [J].
Dubey, JP ;
Hollis, K ;
Romand, S ;
Thulliez, P ;
Kwok, OCH ;
Hungerford, L ;
Anchor, C ;
Etter, D .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1999, 29 (10) :1709-1711
[6]  
DUBEY JP, 1988, J AM VET MED ASSOC, V193, P1259
[7]   Transmission of Neospora caninum between wild and domestic animals [J].
Gondim, LFP ;
McAllister, MM ;
Mateus-Pinilla, NE ;
Pitt, WC ;
Mech, LD ;
Nelson, ME .
JOURNAL OF PARASITOLOGY, 2004, 90 (06) :1361-1365
[8]   Coyotes (Canis latrans) are definitive hosts of Neospora caninum [J].
Gondim, LFP ;
McAllister, MM ;
Pitt, WC ;
Zemlicka, DE .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2004, 34 (02) :159-161
[9]   Application of diagnostic tests in veterinary epidemiologic studies [J].
Greiner, M ;
Gardner, IA .
PREVENTIVE VETERINARY MEDICINE, 2000, 45 (1-2) :43-59
[10]   The p29 and p35 immunodominant antigens of Neospora caninum tachyzoites are homologous to the family of surface antigens of Toxoplasma gondii [J].
Howe, DK ;
Crawford, AC ;
Lindsay, D ;
Sibley, LD .
INFECTION AND IMMUNITY, 1998, 66 (11) :5322-5328
12