Case study: design and implementation of training for scientists deploying to Ebola diagnostic field laboratories in Sierra Leone: October 2014 to February 2016

被引:9
作者
Logue, Christopher H. [1 ]
Lewis, Suzanna M. [1 ]
Lansley, Amber [1 ]
Fraser, Sara [1 ]
Shieber, Clare [1 ]
Shah, Sonal [1 ]
Semper, Amanda [1 ]
Bailey, Daniel [1 ]
Busuttil, Jason [1 ]
Evans, Liz [1 ]
Carroll, Miles W. [1 ]
Silman, Nigel J. [1 ]
Brooks, Tim [1 ]
Shallcross, Jane A. [1 ]
机构
[1] Publ Hlth England, Novel & Dangerous Pathogens Training, Salisbury SP4 0JG, Wilts, England
关键词
training; Ebola virus; field laboratories; diagnostics; outbreak; Sierra Leone; POINT-OF-CARE; VIRUS DISEASE; REAL-TIME; IDENTIFICATION; SURVEILLANCE; OUTBREAK;
D O I
10.1098/rstb.2016.0299
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
As part of the UK response to the 2013-2016 Ebola virus disease (EVD) epidemic in West Africa, Public Health England (PHE) were tasked with establishing three field Ebola virus (EBOV) diagnostic laboratories in Sierra Leone by the UK Department for International Development (DFID). These provided diagnostic support to the Ebola Treatment Centre (ETC) facilities located in Kerry Town, Makeni and Port Loko. The Novel and Dangerous Pathogens (NADP) Training group at PHE, Porton Down, designed and implemented a pre-deployment Ebola diagnostic laboratory training programme for UK volunteer scientists being deployed to the PHE EVD laboratories. Here, we describe the training, workflow and capabilities of these field laboratories for use in response to disease epidemics and in epidemiological surveillance. We discuss the training outcomes, the laboratory outputs, lessons learned and the legacy value of the support provided. We hope this information will assist in the recruitment and training of staff for future responses and in the design and implementation of rapid deployment diagnostic field laboratories for future outbreaks of high consequence pathogens. This article is part of the themed issue 'The 2013-2016 West African Ebola epidemic: data, decision-making and disease control'.
引用
收藏
页数:12
相关论文
共 18 条
[1]  
Alere, 2016, TEST KIT PROD INSTR
[2]  
[Anonymous], 2016, Ebola Situation Report
[3]   Diagnosis of Ebola Virus Disease: Past, Present, and Future [J].
Broadhurst, M. Jana ;
Brooks, Tim J. G. ;
Pollock, Nira R. .
CLINICAL MICROBIOLOGY REVIEWS, 2016, 29 (04) :773-793
[4]   ReEBOV Antigen Rapid Test kit for point-of-care and laboratory-based testing for Ebola virus disease: a field validation study [J].
Broadhurst, Mara Jana ;
Kelly, John Daniel ;
Miller, Ann ;
Semper, Amanda ;
Bailey, Daniel ;
Groppelli, Elisabetta ;
Simpson, Andrew ;
Brooks, Tim ;
Hula, Susan ;
Nyoni, Wilfred ;
Sankoh, Alhaji B. ;
Kanu, Santigi ;
Jalloh, Alhaji ;
Ton, Quy ;
Sarchet, Nicholas ;
George, Peter ;
Perkins, Mark D. ;
Wonderly, Betsy ;
Murray, Megan ;
Pollock, Nira R. .
LANCET, 2015, 386 (9996) :867-874
[5]   Dilemmas in Managing Pregnant Women With Ebola: 2 Case Reports [J].
Caluwaerts, Severine ;
Fautsch, Tessy ;
Lagrou, Daphne ;
Moreau, Michel ;
Camara, Alseny Modet ;
Guenther, Stephan ;
Di Caro, Antonino ;
Borremans, Benny ;
Koundouno, Fara Raymond ;
Bore, Joseph Akoi ;
Logue, Christopher H. ;
Richter, Martin ;
Woelfel, Roman ;
Kuisma, Eeva ;
Kurth, Andreas ;
Thomas, Stephen ;
Burkhardt, Gillian ;
Erland, Elin ;
Lionetto, Fanshen ;
Weber, Patricia Lledo ;
de la Rosa, Olimpia ;
Macpherson, Hassan ;
Van Herp, Michel .
CLINICAL INFECTIOUS DISEASES, 2016, 62 (07) :903-905
[6]   Temporal and spatial analysis of the 2014-2015 Ebola virus outbreak in West Africa [J].
Carroll, Miles W. ;
Matthews, David A. ;
Hiscox, Julian A. ;
Elmore, Michael J. ;
Pollakis, Georgios ;
Rambaut, Andrew ;
Hewson, Roger ;
Garcia-Dorival, Isabel ;
Bore, Joseph Akoi ;
Koundouno, Raymond ;
Abdellati, Said ;
Afrough, Babak ;
Aiyepada, John ;
Akhilomen, Patience ;
Asogun, Danny ;
Atkinson, Barry ;
Badusche, Marlis ;
Bah, Amadou ;
Bate, Simon ;
Baumann, Jan ;
Becker, Dirk ;
Becker-Ziaja, Beate ;
Bocquin, Anne ;
Borremans, Benny ;
Bosworth, Andrew ;
Boettcher, Jan Peter ;
Cannas, Angela ;
Carletti, Fabrizio ;
Castilletti, Concetta ;
Clark, Simon ;
Colavita, Francesca ;
Diederich, Sandra ;
Donatus, Adomeh ;
Duraffour, Sophie ;
Ehichioya, Deborah ;
Ellerbrok, Heinz ;
Fernandez-Garcia, Maria Dolores ;
Fizet, Alexandra ;
Fleischmann, Erna ;
Gryseels, Sophie ;
Hermelink, Antje ;
Hinzmann, Julia ;
Hopf-Guevara, Ute ;
Ighodalo, Yemisi ;
Jameson, Lisa ;
Kelterbaum, Anne ;
Kis, Zoltan ;
Kloth, Stefan ;
Kohl, Claudia ;
Korva, Misa .
NATURE, 2015, 524 (7563) :97-U201
[7]   Ebola virus disease diagnosis by real-time RT-PCR: A comparative study of 11 different procedures [J].
Cherpillod, Pascal ;
Schibler, Manuel ;
Vieille, Gael ;
Cordey, Samuel ;
Mamin, Aline ;
Vetter, Pauline ;
Kaiser, Laurent .
JOURNAL OF CLINICAL VIROLOGY, 2016, 77 :9-14
[8]  
Grolla A, 2005, Bull Soc Pathol Exot, V98, P205
[9]   The Use of a Mobile Laboratory Unit in Support of Patient Management and Epidemiological Surveillance during the 2005 Marburg Outbreak in Angola [J].
Grolla, Allen ;
Jones, Steven M. ;
Fernando, Lisa ;
Strong, James E. ;
Stroeher, Ute ;
Moeller, Peggy ;
Paweska, Janusz T. ;
Burt, Felicity ;
Pablo Palma, Pedro ;
Sprecher, Armand ;
Formenty, Pierre ;
Roth, Cathy ;
Feldmann, Heinz .
PLOS NEGLECTED TROPICAL DISEASES, 2011, 5 (05)
[10]   Real-time, portable genome sequencing for Ebola surveillance [J].
Quick, Joshua ;
Loman, Nicholas J. ;
Duraffour, Sophie ;
Simpson, Jared T. ;
Severi, Ettore ;
Cowley, Lauren ;
Bore, Joseph Akoi ;
Koundouno, Raymond ;
Dudas, Gytis ;
Mikhail, Amy ;
Ouedraogo, Nobila ;
Afrough, Babak ;
Bah, Amadou ;
Baum, Jonathan H. J. ;
Becker-Ziaja, Beate ;
Boettcher, Jan Peter ;
Cabeza-Cabrerizo, Mar ;
Camino-Sanchez, Alvaro ;
Carter, Lisa L. ;
Doerrbecker, Juliane ;
Enkirch, Theresa ;
Garcia-Dorival, Isabel ;
Hetzelt, Nicole ;
Hinzmann, Julia ;
Holm, Tobias ;
Kafetzopoulou, Liana Eleni ;
Koropogui, Michel ;
Kosgey, Abigael ;
Kuisma, Eeva ;
Logue, Christopher H. ;
Mazzarelli, Antonio ;
Meisel, Sarah ;
Mertens, Marc ;
Michel, Janine ;
Ngabo, Didier ;
Nitzsche, Katja ;
Pallasch, Elisa ;
Patrono, Livia Victoria ;
Portmann, Jasmine ;
Repits, Johanna Gabriella ;
Rickett, Natasha Y. ;
Sachse, Andreas ;
Singethan, Katrin ;
Vitoriano, Ines ;
Emanaberhan, Rahel L. Y. ;
Zekeng, Elsa G. ;
Racine, Trina ;
Bello, Alexander ;
Sall, Amadou Alpha ;
Faye, Ousmane .
NATURE, 2016, 530 (7589) :228-+