A challenge in anaerobic digestion of swine wastewater: recalcitrance and enhanced-degradation of dietary fibres

Dietary fibres are main substances in the pig's feed. Because of the recalcitrance, they could enter swine wastewater and become a serious obstruction factor for the anaerobic digestion process. In this work, three dietary fibres abundant in pig feedstocks: Wheat Bran Fibre (WBF), Alfalfa Fibre (AF) and Rice Chaff Fibre (RCF) were chosen and their anaerobic degradability was determined. The results showed that the biochemical methane potential in 10 days (BMP10) of WBF, AF and RCF was 258, 176 and 86 mL/g-VS, respectively. The size, purity, crystallinity, and lignin coating in particular, were found having influences on the anaerobic biodegradability of dietary fibres. To surprise, a negative rather than positive effect was observed for the direct addition of extraneous cellulase into the anaerobic digestion systems, leading to a longer lag time and a smaller BMP10. The enhancement was achieved for the addition of extraneous bacteria in the form of anaerobic granular sludge (AnGS), shortening the lag time of WBF and AF by 36% and 13%, respectively. By high-throughput sequencing analysis, abundant protein and amino acids degraders found in anaerobic activated sludge (AnAS) could degrade the exogenous enzymes. Abundant members affiliated to the family Anaerolineaceae, and Syntrophobacteraceae in AnGS, related to the cellulolytic and syntrophic activity respectively, probably contribute to the acceleration effect of AnGS.