Structure of a viscous exopolysaccharide produced by Lactobacillus helveticus K16

被引:29
作者
Yang, ZN
Staaf, M
Huttunen, E
Widmalm, G [1 ]
机构
[1] Univ Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden
[2] Univ Helsinki, Dept Food Technol, FIN-00014 Helsinki, Finland
基金
芬兰科学院;
关键词
Lactobacillus helveticus; exopolysaccharide; viscous; NMR;
D O I
10.1016/S0008-6215(00)00201-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A viscous extracellular polysaccharide produced by Lactobacillus helveticus K16 has been investigated. Sugar and methylation analysis, H-1 and C-13 NMR spectroscopy revealed that the polysaccharide is composed of a hexasaccharide repeating unit. The sequence of sugar residues was determined by use of two-dimensional nuclear Overhauser effect spectroscopy and heteronuclear multiple bond connectivity experiments. The structure of the repeating unit of the exopolysaccharide from L. helveticus K16 is as follows: [GRAPHICS] (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:465 / 469
页数:5
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