Nanoparticle-Based Delivery System for Application of siRNA In Vivo

被引：78

作者：

Wang, Yan ^{[1
,2
]}

Li, Zhiguo ^{[1
]}

Han, Yee ^{[3
]}

Liang, Leo Hwa ^{[1
,3
]}

Ji, Aimin ^{[1
]}

机构：

[1] So Med Univ, Zhujiang Hosp, Guangzhou, Guangdong, Peoples R China

[2] Inst Bioengn & Nanotechnol, Singapore, Singapore

[3] Natl Univ Singapore, Div Bioengn, Singapore 117548, Singapore

来源：

CURRENT DRUG METABOLISM | 2010年 / 11卷 / 02期

关键词：

RNAi; small interfering RNA; delivery system; nanoparticles; efficacy; safety; clinical trials; SMALL INTERFERING RNA; WALL CARBON NANOTUBES; DOUBLE-STRANDED-RNA; GENE-TRANSFER; PULMONARY TOXICITY; ENDOGENOUS SIRNAS; TARGETED DELIVERY; DRUG-DELIVERY; PLASMID DNA; TRANSFECTION EFFICIENCY;

D O I：

10.2174/138920010791110863

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small interfering RNAs (siRNAs) silence the expression of specific target genes by mediating RNA interference (RNAi) in mammalian cells. siRNAs have not only been widely used as a valuable tool for functional genomics research, but they also have demonstrated great potential in biomedical therapeutic applications for diseases caused by abnormal gene overexpression or mutation. One of the most important issues to overcome before full clinical application is the development of effective administration methods for siRNAs to the target tissue or cells in vivo, which is highly dependent on the delivery system. Currently, there are two major kinds of in vivo delivery systems: viral or nonviral. As one of the nonviral carrier systems, nanoparticles, combinations of liposomes and cationic polymer complexes, have exhibited improved in vivo stability, target specificity, and cell/tissue uptake and internalization of the encapsulated RNAi oligos, which result in more effective silencing with less cellular toxicity and immune stimulation. This review will discuss the latest advancements in nanoparticle-mediated RNAi delivery systems, including nano-materials, preparation, and characteristics. In conjunction, the clinical trial cases related to the nanoparticle-siRNA complexes will be highlighted. The safety issues of nanoparticles used in vivo will also be mentioned. Finally, this review will summarize the perspectives for future applications of nanoparticle-mediated RNAi delivery systems.

引用

页码：182 / 196

页数：15

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