Comparative study of different alcoholate pretreatments for enhanced enzymatic hydrolysis of sugarcane bagasse

被引：17
|
作者：
Huang, Qing [1 ]
Yan, Qiuli [1 ]
Fu, Jing [1 ]
Lv, Xiaojing [1 ]
Xiong, Chunjiang [2 ]
Lin, Jianghai [1 ]
Liu, Zehuan [1 ]
机构：
[1] Jinan Univ, Inst Life & Hlth Engn, Coll Life Sci & Technol, Res Ctr Mol Biol, Guangzhou 510632, Guangdong, Peoples R China
[2] Guangdong Qizhi Biotechnol Co Ltd, Dept Res & Dev, Guangzhou, Guangdong, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2016年 / 211卷
关键词：
Alcoholate pretreatment; Response surface methodology; Enzymatic hydrolysis; Synergism; Sugarcane bagasse; ETHANOL-PRODUCTION; LIGNOCELLULOSIC BIOMASS; BIOETHANOL PRODUCTION; SODIUM ETHOXIDE; ACID; XYLANASE; ALKALI; SACCHARIFICATION; FERMENTATION; OPTIMIZATION;
D O I：
10.1016/j.biortech.2016.03.067
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Pretreatment of sugarcane bagasse (SCB) with alcoholates, sodium methoxide (CH3ONa), potassium methoxide (CH3OK) and sodium ethoxide (C2H5ONa), was investigated. Analyses of lignocellulose composition and enzymatic saccharification indicated that C2H5ONa showed the highest enzymatic efficiency of 102.1%. The response surface optimization of C2H5ONa pretreatment showed that under optimal conditions (4% of C2H5ONa, 121 degrees C, 1 h), 65.4% of lignin was removed and the enzymatic efficiency reached 105.2%. Hydrolysis of SCB with cellulases and xylanase at a ratio of 4:1 showed the strongest synergism with reducing sugar production of 21 g/L and conversion rates of cellulose and xylan reaching 110.4% and 94.5%, respectively. These results indicated that C2H5ONa is a promising alkali to pretreat SCB and the synergism between cellulases and xylanase has a significant effect on enzymatic saccharification of the pretreated SCB. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：464 / 471
页数：8
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