Insights into nucleic acid reactivity through gas-phase experimental and computational studies

被引：38

作者：

Lee, JK ^{[1
]}

机构：

[1] Rutgers State Univ, Dept Chem & Biol Chem, Piscataway, NJ 08854 USA

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2005年 / 240卷 / 03期

关键词：

nucleic acids; RNA DNA; enzyme nucleobase; acidity; proton affinity; URACIL-DNA GLYCOSYLASE; OROTIDINE MONOPHOSPHATE DECARBOXYLASE; PROTON AFFINITY; BIOLOGICAL IMPLICATIONS; ADENINE-DERIVATIVES; PROFICIENT ENZYME; MASS-SPECTROMETRY; BASE-EXCISION; MECHANISM; SITES;

D O I：

10.1016/j.ijms.2004.09.020

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Accurate measurements of the acidities and basicities of nucleic bases and nucleic base derivatives is essential for understanding, issues of fundamental importance in biological systems. Hydrogen bonding modulates recognition of DNA and RNA bases. and the interaction energy between two bonded complementary nucleobases is dependent on the intrinsic basicity and acidity of the acceptor and donor groups. In addition, understanding the intrinsic reactivity of nucleic bases can shed light on key biosynthetic mechanisms for which nucleobases are substrates. In this review, we highlight advances in our lab toward understanding the fundamental reactivity of DNA and RNA. In particular. we focus on our investigation of the gas phase acidities and basicities of natural and unnatural nucleobases, and the implications of our results for the mechanisms of nucleotide biosynthetic and repair enzymes. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：261 / 272

页数：12

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