Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen

被引：57

作者：

Chapman, Michael R. ^{[1
,2
]}

Cosgrove, Sebastian C. ^{[4
]}

Turner, Nicholas J. ^{[4
]}

Kapur, Nikil ^{[3
]}

Blacker, A. John ^{[1
,2
]}

机构：

[1] Univ Leeds, Sch Chem, Woodhouse Lane, Leeds LS2 9JT, W Yorkshire, England

[2] Univ Leeds, Sch Chem & Proc Engn, Inst Proc Res & Dev, Woodhouse Lane, Leeds LS2 9JT, W Yorkshire, England

[3] Univ Leeds, Sch Mech Engn, Woodhouse Lane, Leeds LS2 9JT, W Yorkshire, England

[4] Manchester Inst Biotechnol, 131 Princess St, Manchester M1 7DN, Lancs, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2018年 / 57卷 / 33期

基金：

英国生物技术与生命科学研究理事会;

关键词：

biocatalysis; continuous flow; flow reactors; oxidation; space-time yields; GALACTOSE-OXIDASE; GREEN CHEMISTRY; DERACEMIZATION; CATALYSTS; KINETICS; REACTOR; TANDEM; WATER;

D O I：

10.1002/anie.201803675

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a simple, mild, and synthetically clean approach to accelerate the rate of enzymatic oxidation reactions by a factor of up to 100 when compared to conventional batch gas/liquid systems. Biocatalytic decomposition of H2O2 is used to produce a soluble source of O-2 directly in reaction media, thereby enabling the concentration of aqueous O-2 to be increased beyond equilibrium solubility under safe and practical conditions. To best exploit this method, a novel flow reactor was developed to maximize productivity (g product L(-1)h(-1)). This scalable benchtop method provides a distinct advantage over conventional bio-oxidation in that no pressurized gas or specialist equipment is employed. The method is general across different oxidase enzymes and compatible with a variety of functional groups. These results culminate in record space-time yields for bio-oxidation.

引用

页码：10535 / 10539

页数：5

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