Whole-field optically sectioned fluorescence lifetime imaging

被引:46
作者
Cole, MJ
Siegel, J
Webb, SED
Jones, R
Dowling, K
French, PMW
Lever, MJ
Sucharov, LOD
Neil, MAA
Juskaitis, R
Wilson, T
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Femtosecond Opt Grp, London SW7 2BW, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol & Med Syst, London SW7 2BY, England
[3] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
关键词
D O I
10.1364/OL.25.001361
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We describe a novel three-dimensional fluorescence lifetime imaging microscope that exploits structured illumination to achieve whole-field sectioned fluorescence lifetime images with a spatial resolution of a few micrometers. (C) 2000 Optical Society of America.
引用
收藏
页码:1361 / 1363
页数:3
相关论文
共 13 条
[1]  
[Anonymous], PRINCIPLES FLUORESEN
[2]  
CUBBEDU R, 1993, OPT ENG, V32, P320
[3]   Fluorescence lifetime imaging with picosecond resolution for biomedical applications [J].
Dowling, K ;
Dayel, MJ ;
Lever, MJ ;
French, PMW ;
Hares, JD ;
Dymoke-Bradshaw, AKL .
OPTICS LETTERS, 1998, 23 (10) :810-812
[4]  
DOWLING K, 1999, J MOD OPT, V46
[5]  
Hanley QS, 2000, J MICROSC-OXFORD, V197, P5, DOI 10.1046/j.1365-2818.2000.00665.x
[6]   Fluorescence lifetime imaging using a diode-pumped all-solid-state laser system [J].
Jones, R ;
Dowling, K ;
Cole, MJ ;
Parsons-Karavassilis, D ;
Lever, MJ ;
French, PMW ;
Hares, JD ;
Dymoke-Bradshaw, AKL .
ELECTRONICS LETTERS, 1999, 35 (04) :256-258
[7]   Wide-field optically sectioning fluorescence microscopy with laser illumination [J].
Neil, M.A.A. ;
Squire, A. ;
Juškaitis, R. ;
Bastiaens, P.I.H. ;
Wilson, T. .
Journal of Microscopy, 2000, 197 (01) :1-4
[8]   Method of obtaining optical sectioning by using structured light in a conventional microscope [J].
Neil, MAA ;
Juskaitis, R ;
Wilson, T .
OPTICS LETTERS, 1997, 22 (24) :1905-1907
[9]   Rapid acquisition, analysis, and display of fluorescence lifetime-resolved images for real-time applications [J].
Schneider, PC ;
Clegg, RM .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1997, 68 (11) :4107-4119
[10]   Three dimensional image restoration in fluorescence lifetime imaging microscopy [J].
Squire, A ;
Bastiaens, PIH .
JOURNAL OF MICROSCOPY, 1999, 193 :36-49