In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites

被引：50

作者：

Grey, Corinne ^{[1
]}

Clement, Julie A. J. ^{[1
]}

Buard, Jerome ^{[1
]}

Leblanc, Benjamin ^{[2
]}

Gut, Ivo ^{[3
,4
]}

Gut, Marta ^{[3
,4
]}

Duret, Laurent ^{[5
]}

de Massy, Bernard ^{[1
]}

机构：

[1] Univ Montpellier, CNRS, UMR9002, Inst Genet Humaine, F-34396 Montpellier 05, France

[2] Univ Copenhagen, BRIC, DK-2200 Copenhagen, Denmark

[3] BIST, CRG, CNAG CRG, Barcelona 08028, Spain

[4] UPF, Barcelona 08003, Spain

[5] Univ Claude Bernard, Univ Lyon, CNRS, Lab Biometrie & Biol Evolut,UMR 5558, F-69100 Villeurbanne, France

来源：

GENOME RESEARCH | 2017年 / 27卷 / 04期

基金：

欧洲研究理事会;

关键词：

STRAND BREAK FORMATION; MEIOTIC RECOMBINATION INITIATION; REPAIR PATHWAY CHOICE; H3; LYSINE; 36; HOMOLOGOUS RECOMBINATION; CHROMOSOME SYNAPSIS; GENE CONVERSION; MOUSE GENOME; DNA; MICE;

D O I：

10.1101/gr.217240.116

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis.

引用

页码：580 / 590

页数：11