Study of bed homogenity of methacrylate-based monolithic columns for micro-HPLC and CEC

The preparation of polymeric monolithic (rod) columns for micro-HPLC and capillary electro-chromatography (CEC) was described. Monolithic micro-columns were synthesized from methacrylates possesing different functional groups such as: butyl, laury, octadecyl and isobornyl. Special attention was paid on the modification of quartz capillary wall and before formations of capillary column bed as a result of polymerization. The evaluation of homogeneity and quality of formed beds was performed using scanning electron microscopy (SEM), porosimetry and electrochromatography and/or micro-HPLC. Good synthesis reproducibility, high homogeneity of formed beds and also high efficiency (N = 90 000 plates m(-1) for micro-HPLC and N = 140 000 plates m(-1) for CEC) were obtained.