Multiplexed, quantitative serological profiling of COVID-19 from blood by a point-of-care test

被引：41

作者：

Heggestad, Jacob T. ^{[1
]}

Kinnamon, David S. ^{[1
]}

Olson, Lyra B. ^{[2
]}

Liu, Jason ^{[1
]}

Kelly, Garrett ^{[1
]}

Wall, Simone A. ^{[1
]}

Oshabaheebwa, Solomon ^{[1
]}

Quinn, Zachary ^{[1
]}

Fontes, Cassio M. ^{[1
]}

Joh, Daniel Y. ^{[1
,3
]}

Hucknall, Angus M. ^{[1
]}

Pieper, Carl ^{[4
]}

Anderson, Jack G. ^{[5
]}

Naqvi, Ibtehaj A. ^{[6
]}

Chen, Lingye ^{[7
]}

Que, Loretta G. ^{[7
]}

Oguin, Thomas, III ^{[8
,9
]}

Nair, Smita K. ^{[6
,10
]}

Sullenger, Bruce A. ^{[1
,6
,11
]}

Woods, Christopher W. ^{[5
,8
,9
]}

Burke, Thomas W. ^{[5
]}

Sempowski, Gregory D. ^{[8
,9
]}

Kraft, Bryan D. ^{[7
]}

Chilkoti, Ashutosh ^{[1
]}

机构：

[1] Duke Univ, Pratt Sch Engn, Dept Biomed Engn, Durham, NC 27708 USA

[2] Duke Univ, Dept Pharmacol & Canc Biol, Duke Med Scientist Training Program, Sch Med, Durham, NC 27710 USA

[3] Duke Univ, Dept Surg, Div Plast Maxillofacial & Oral Surg, Med Ctr, Durham, NC 27710 USA

[4] Duke Univ, Dept Biostat & Bioinformat, Med Ctr, Durham, NC 27708 USA

[5] Duke Univ, Ctr Appl Genom & Precis Med, Dept Med, Durham, NC 27710 USA

[6] Duke Univ, Dept Surg, Sch Med, Durham, NC 27710 USA

[7] Duke Univ, Dept Med, Div Pulm Allergy & Crit Care Med, Med Ctr, Durham, NC 27710 USA

[8] Duke Univ, Sch Med, Dept Med, Durham, NC 27710 USA

[9] Duke Univ, Sch Med, Duke Human Vaccine Inst, Durham, NC 27710 USA

[10] Duke Univ, Sch Med, Dept Neurosurg & Pathol, Durham, NC 27710 USA

[11] Duke Univ, Dept Pharmacol & Canc Biol, Sch Med, Durham, NC 27710 USA

来源：

SCIENCE ADVANCES | 2021年 / 7卷 / 26期

基金：

美国国家科学基金会;

关键词：

ANTIBODY;

D O I：

10.1126/sciadv.abg4901

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Highly sensitive, specific, and point-of-care (POC) serological assays are an essential tool to manage coronavirus disease 2019 (COVID-19). Here, we report on a microfluidic POC test that can profile the antibody response against multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens-spike S1 (S1), nucleocapsid (N), and the receptor binding domain (RBD)-simultaneously from 60.l of blood, plasma, or serum. We assessed the levels of antibodies in plasma samples from 31 individuals (with longitudinal sampling) with severe COVID-19, 41 healthy individuals, and 18 individuals with seasonal coronavirus infections. This POC assay achieved high sensitivity and specificity, tracked seroconversion, and showed good concordance with a live virus microneutralization assay. We can also detect a prognostic biomarker of severity, IP-10 (interferon-gamma-induced protein 10), on the same chip. Because our test requires minimal user intervention and is read by a handheld detector, it can be globally deployed to combat COVID-19.

引用

页数：13

共 51 条

[1] Preliminary Identification of Potential Vaccine Targets for the COVID-19 Coronavirus (SARS-CoV-2) Based on SARS-CoV Immunological Studies [J].

Ahmed, Syed Faraz ;

Quadeer, Ahmed A. ;

McKay, Matthew R. .

VIRUSES-BASEL, 2020, 12 (03)

[2]

Amanat F, 2020, NAT MED, V26, P1033, DOI [10.1038/s41591-020-0913-5, 10.1101/2020.03.17.20037713]

[3]

Armbruster D. A., 2008, CLIN BIOCHEM REV, V29 Suppl1, pS49, DOI DOI 10.3390/S20236922

[4] Distinct Early Serological Signatures Track with SARS-CoV-2 Survival [J].

Atyeo, Caroline ;

Fischinger, Stephanie ;

Zohar, Tomer ;

Slein, Matthew D. ;

Burke, John ;

Loos, Carolin ;

McCulloch, Denise J. ;

Newman, Kira L. ;

Wolf, Caitlin ;

Yu, Jingyou ;

Shuey, Kiel ;

Feldman, Jared ;

Hauser, Blake Marie ;

Caradonna, Tim ;

Schmidt, Aaron G. ;

Suscovich, Todd J. ;

Linde, Caitlyn ;

Cai, Yongfei ;

Barouch, Dan ;

Ryan, Edward T. ;

Charles, Richelle C. ;

Lauffenburger, Douglas ;

Chu, Helen ;

Alter, Galit .

IMMUNITY, 2020, 53 (03) :524-+

[5] Diagnostic accuracy of serological tests for covid-19: systematic review and meta-analysis [J].

Bastos, Mayara Lisboa ;

Tavaziva, Gamuchirai ;

Abidi, Syed Kunal ;

Campbell, Jonathon R. ;

Haraoui, Louis -Patrick ;

Johnston, James C. ;

Lan, Zhiyi ;

Law, Stephanie ;

MacLean, Emily ;

Trajman, Anete ;

Menzies, Dick ;

Benedetti, Andrea ;

Khan, Faiz Ahmad .

BMJ-BRITISH MEDICAL JOURNAL, 2020, 370

[6] Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus [J].

Berry, JD ;

Jones, S ;

Drebot, MA ;

Andonov, A ;

Sabara, M ;

Yuan, XY ;

Weingartl, H ;

Fernando, L ;

Marszal, P ;

Gren, J ;

Nicolas, B ;

Andonova, M ;

Ranada, F ;

Gubbins, MJ ;

Ball, TB ;

Kitching, P ;

Li, Y ;

Kabani, A ;

Plummer, F .

JOURNAL OF VIROLOGICAL METHODS, 2004, 120 (01) :87-96

[7] Serology for SARS-CoV-2: Apprehensions, opportunities, and the path forward [J].

Bryant, Juliet E. ;

Azman, Andrew S. ;

Ferrari, Matthew J. ;

Arnold, Benjamin F. ;

Boni, Maciej F. ;

Boum, Yap ;

Hayford, Kyla ;

Luquero, Francisco J. ;

Mina, Michael J. ;

Rodriguez-Barraquer, Isabel ;

Wu, Joseph T. ;

Wade, Djibril ;

Vernet, Guy ;

Leung, Daniel T. .

SCIENCE IMMUNOLOGY, 2020, 5 (47)

[8] Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia [J].

Chu, Daniel K. W. ;

Pan, Yang ;

Cheng, Samuel M. S. ;

Hui, Kenrie P. Y. ;

Krishnan, Pavithra ;

Liu, Yingzhi ;

Ng, Daisy Y. M. ;

Wan, Carrie K. C. ;

Yang, Peng ;

Wang, Quanyi ;

Peiris, Malik ;

Poon, Leo L. M. .

CLINICAL CHEMISTRY, 2020, 66 (04) :549-555

[9] Nucleocapsid Protein Recruitment to Replication-Transcription Complexes Plays a Crucial Role in Coronaviral Life Cycle [J].

Cong, Yingying ;

Ulasli, Mustafa ;

Schepers, Hein ;

Mauthe, Mario ;

V'kovski, Philip ;

Kriegenburg, Franziska ;

Thiel, Volker ;

de Haan, Cornelis A. M. ;

Reggiori, Fulvio .

JOURNAL OF VIROLOGY, 2020, 94 (04)

[10]

Crowther Jr, 2000, METH MOL B, V149, pV

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