Anti-inflammatory and anti-angiogenic activities of a purified polysaccharide from flesh of Cipangopaludina chinensis

被引：48

作者：

Xiong, Qingping ^{[1
,3
]}

Hao, Hairong ^{[2
]}

He, Lian ^{[4
]}

Jing, Yi ^{[1
]}

Xu, Tingting ^{[1
,3
]}

Chen, Jing ^{[3
]}

Zhang, Haijiang ^{[1
]}

Hu, Tao ^{[1
,3
]}

Zhang, Qianghua ^{[1
]}

Yang, Xue ^{[5
]}

Yuan, Jun ^{[1
,3
]}

Huang, Yu ^{[1
]}

机构：

[1] Huaiyin Inst Technol, Sch Chem Engn, Key Lab Med Exploitat & Utilizat Reg Resources, Huaian 223003, Jiangsu, Peoples R China

[2] Xuzhou Med Univ, Huaian Hosp, Huaian 223002, Jiangsu, Peoples R China

[3] Huaiyin Inst Technol, Key Lab Palygorskite Sci & Appl Technol Jiangsu P, Huaian 223003, Jiangsu, Peoples R China

[4] Guangdong Food & Drug Vocat Coll, Guangzhou 510520, Guangdong, Peoples R China

[5] Qilu Univ Technol, Sch Bioengn, Jinan 250353, Shandong, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2017年 / 176卷

基金：

中国国家自然科学基金;

关键词：

Cipangopaludina chinensis; Edible flesh; Polysaccharide; Anti-inflammatory activity; Anti-angiogenic activity; CANCER; INFLAMMATION; THERAPY; GROWTH; INHIBITION; MIGRATION; PATHWAY; ROLES; CELLS;

D O I：

10.1016/j.carbpol.2017.08.073

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The purpose of this paper was to investigate the anti-inflammatory and anti-angiogenic activities of a purified polysaccharide (CCPSn) from flesh of Cipangopaludina chinensis in lipopolysaccharide (LPS) activated RAW264.7 macrophages and human umbilical vein endothelial cells (HUVECs), respectively. Anti-inflammatory results showed that CCPS not only greatly decreased the pro -/anti-inflammatory cytokine secretion ratios, including TNF-alpha/IL-10, IL-6/IL-10 and IL-1 beta/IL-10, but also reduced release levels of nitric oxide (NO) and prostaglandin E-2 (PGE(2)), and suppressed expressions of cyclooxygenase2 (COX2) and inducible nitric oxide synthase (iNOS). The results from anti-angiogenic activities revealed that proliferation, migration, tube formation and vascular endothelial growth factor (VEGF) of HUVECs were significantly inhibited by CCPSn treatment. Even more remarkable was the fact that anti-inflammatory and anti-angiogenic activities of CCPSn exhibited a clear dose response manner, and these activities were irrelevant to the cytotoxicities of CCPSn to RAW264.7 macrophages and HUVECs. These results indicated that CCPSn possessed strong anti-inflammatory and anti-angiogenic activities.

引用

页码：152 / 159

页数：8

共 28 条

[11] Tea catechins inhibit angiogenesis endothelial cell growth, migration in vitro, measured by human and tube formation, through inhibition of VEGF receptor binding [J].