Passive immunity to porcine epidemic diarrhea virus following immunization of pregnant gilts with a recombinant orf virus vector expressing the spike protein

被引：29

作者：

Joshi, Lok R. ^{[1
,2
]}

Okda, Faten A. ^{[1
]}

Singrey, Aaron ^{[1
,2
]}

Maggioli, Mayara F. ^{[1
,2
]}

Faccin, Tatiane C. ^{[1
,3
]}

Fernandes, Maureen H. V. ^{[1
,2
]}

Hain, Kyle S. ^{[1
]}

Dee, Scott ^{[4
]}

Bauermann, Fernando V. ^{[1
,2
]}

Nelson, Eric A. ^{[1
,2
]}

Diel, Diego G. ^{[1
,2
]}

机构：

[1] Dept Vet & Biomed Sci, Anim Dis Res & Diagnost Lab, 1155 North Campus Dr,Box 2175, Brookings, SD 57007 USA

[2] South Dakota State Univ, Ctr Biol Res & Commercializat, Brookings, SD 57007 USA

[3] Univ Fed Santa Maria, Programa Posgrad Med Vet, BR-97105900 Santa Maria, RS, Brazil

[4] Pipestone Vet Serv, Pipestone Appl Res, Pipestone, MN 56164 USA

来源：

ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 09期

基金：

美国食品与农业研究所;

关键词：

TRANSMISSIBLE GASTROENTERITIS; SWINE; SERUM; MILK; COLOSTRUM; VACCINES;

D O I：

10.1007/s00705-018-3855-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Passive immunity is critical for protection of neonatal piglets against porcine epidemic diarrhea virus (PEDV). Here, we investigated the immunogenicity of an orf virus (ORFV) vector expressing the full-length spike (S) protein of PEDV (ORFV-PEDV-S) in pregnant gilts and its ability to confer passive immunity and protection in piglets. Three doses of ORFV-PEDV-S were given to two groups of PEDV-negative pregnant gilts, with the last dose being administered two weeks prior to farrowing. One of the two groups immunized with the ORFV-PEDV-S recombinant virus was also exposed to live PEDV orally on day 31 post-immunization (pi). Antibody responses were assessed in serum, colostrum and milk of immunized gilts, and passive transfer of antibodies was evaluated in piglet sera. The protective efficacy of ORFV-PEDV-S was evaluated after challenge of the piglets with PEDV. PEDV-specific IgG, IgA and neutralizing antibody (NA) responses were detected in ORFV-PEDV-S-immunized and ORFV-PEDV-S-immunized/PEDV-exposed gilts. PEDV NA, IgG and IgA were detected in the serum of piglets born to immunized gilts, demonstrating the transfer of antibodies through colostrum and milk. Piglets born to immunized gilts showed reduced morbidity and a marked reduction in mortality after PEDV challenge in comparison to control piglets. Piglets born to gilts that received ORFV-PEDV-S and were exposed to live PEDV showed stronger NA responses and lower clinical scores when compared to piglets born to gilts immunized with ORFV-PEDV-S alone. These results demonstrate the potential of ORFV as a vaccine delivery platform capable of eliciting passive immunity against PEDV.

引用

页码：2327 / 2335

页数：9

共 18 条

[1] ANTIBODY-RESPONSES IN SERUM, COLOSTRUM, AND MILK OF SWINE AFTER INFECTION OR VACCINATION WITH TRANSMISSIBLE GASTROENTERITIS VIRUS [J].