Catalytic efficiency and kcat/KM:: a useful comparator?

被引：236

作者：

Eisenthal, Robert

Danson, Michael J.

Hough, David W. ^{[1
]}

机构：

[1] Univ Bath, Ctr Extremophile Res, Bath BA2 7AY, Avon, England

[2] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England

来源：

TRENDS IN BIOTECHNOLOGY | 2007年 / 25卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/j.tibtech.2007.03.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ratio k(cat)/K-M - often referred to as the 'specificity constant' - is a useful index for comparing the relative rates of an enzyme acting on alternative, competing substrates. However, an alternative description, 'catalytic efficiency', is frequently used, and on occasions misused, to compare the reactivity of two enzymes acting on the same substrate. Here, we highlight the pitfalls in using k(cat)/K-M to compare the catalytic effectiveness of enzymes.

引用

页码：247 / 249

页数：3