The perils of PCR: can we accurately 'correct' antimalarial trials?

被引：42
作者：
Juliano, Jonathan J. [4 ]
Gadalla, Nahla [2 ,3 ]
Sutherland, Colin J. [2 ,3 ]
Meshnick, Steven R. [1 ]
机构：
[1] Univ N Carolina, Dept Epidemiol, Gillings Sch Global Publ Hlth, Chapel Hill, NC 27599 USA
[2] Hosp Trop Dis, Dept Clin Parasitol, London WC1E 6JB, England
[3] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England
[4] Univ N Carolina, Sch Med, Dept Med, Div Infect Dis, Chapel Hill, NC 27599 USA
来源：
TRENDS IN PARASITOLOGY | 2010年 / 26卷 / 03期
关键词：
PLASMODIUM-FALCIPARUM MALARIA; HETERODUPLEX TRACKING ASSAY; POLYMERASE-CHAIN-REACTION; CLINICAL-TRIALS; ARTEMETHER-LUMEFANTRINE; POPULATION-DYNAMICS; INFECTIONS; PARASITES; EFFICACY; RECRUDESCENCE;
D O I：
10.1016/j.pt.2009.12.007
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates. The epidemiological, biological and technical limitations of PCR correction and how this can lead to misclassification in drug trial outcomes are underappreciated. This article considers these limitations and proposes a framework for reporting, interpreting and improving PCR correction of antimalarial trials.
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收藏
页码：119 / 124
页数：6
相关论文
共 46 条
[1]   Revisiting the Design of Phase III Clinical Trials of Antimalarial Drugs for Uncomplicated Plasmodium falciparum Malaria [J].
Borrmann, Steffen ;
Peto, Tim ;
Snow, Robert W. ;
Gutteridge, Win ;
White, Nicholas J. .
PLOS MEDICINE, 2008, 5 (11) :1546-1552
[2]   Cross-species interactions between malaria parasites in humans [J].
Bruce, MC ;
Donnelly, CA ;
Alpers, MP ;
Galinski, MR ;
Barnwell, JW ;
Walliker, D ;
Day, KP .
SCIENCE, 2000, 287 (5454) :845-848
[3]   Massively parallel pyrosequencing in HIV research [J].
Bushman, Frederic D. ;
Hoffmann, Christian ;
Ronen, Keshet ;
Malani, Nirav ;
Minkah, Nana ;
Rose, Heather Marshall ;
Tebas, Pablo ;
Wang, Gary P. .
AIDS, 2008, 22 (12) :1411-1415
[4]   The use of genotyping in antimalarial clinical trials: a systematic review of published studies from 1995-2005 [J].
Collins, William J. ;
Greenhouse, Bryan ;
Rosenthal, Philip J. ;
Dorsey, Grant .
MALARIA JOURNAL, 2006, 5 (1)
[5]  
Curran J.M., 2005, LAW PROBABILITY & RISK, V4, P115, DOI [10.1093/lpr/mgi009, 10.1093/1pr/mgi009, DOI 10.1093/LPR/MGI009]
[6]   Rapid turnover of Plasmodium falciparum populations in asymptomatic individuals living in a high transmission area [J].
Daubersies, P ;
SallenaveSales, S ;
Magne, S ;
Trape, JF ;
Contamin, H ;
Fandeur, T ;
Rogier, C ;
MercereauPuijalon, O ;
Druilhe, P .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1996, 54 (01) :18-26
[7]   Homogeneous quasispecies in 16 out of 17 individuals during very early HIV-1 primary infection [J].
Delwart, E ;
Magierowska, M ;
Royz, M ;
Foley, B ;
Peddada, L ;
Smith, R ;
Heldebrant, C ;
Conrad, A ;
Busch, M .
AIDS, 2002, 16 (02) :189-195
[8]   Artemisinin Resistance in Plasmodium falciparum Malaria. [J].
Dondorp, Arjen M. ;
Nosten, Francois ;
Yi, Poravuth ;
Das, Debashish ;
Phyo, Aung Phae ;
Tarning, Joel ;
Lwin, Khin Maung ;
Ariey, Frederic ;
Hanpithakpong, Warunee ;
Lee, Sue J. ;
Ringwald, Pascal ;
Silamut, Kamolrat ;
Imwong, Mallika ;
Chotivanich, Kesinee ;
Lim, Pharath ;
Herdman, Trent ;
An, Sen Sam ;
Yeung, Shunmay ;
Singhasivanon, Pratap ;
Day, Nicholas P. J. ;
Lindegardh, Niklas ;
Socheat, Duong ;
White, Nicholas J. .
NEW ENGLAND JOURNAL OF MEDICINE, 2009, 361 (05) :455-467
[9]   Extensive dynamics of Plasmodium falciparum densities, stages and genotyping profiles [J].
Farnert, Anna ;
Lebbad, Marianne ;
Faraja, Lea ;
Rooth, Ingegerd .
MALARIA JOURNAL, 2008, 7 (1)
[10]   Can estimates of antimalarial efficacy from field studies be improved? [J].
Gatton, Michelle L. ;
Cheng, Qin .
TRENDS IN PARASITOLOGY, 2008, 24 (02) :68-73
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