Near-visible ultraviolet light induces a novel ubiquitous calcium-permeable cation current in mammalian cell lines

被引:44
作者
Mendez, F [1 ]
Penner, R [1 ]
机构
[1] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1998年 / 507卷 / 02期
关键词
D O I
10.1111/j.1469-7793.1998.365bt.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. We studied the immediate and short-term effects of UV light in the near-visible range at the cellular and membrane level using the whole-cell patch-damp technique in combination with digital fluorescence imaging. 2. Illumination with monochromatic UVA light (340-380 nm) induced a sustained nonsaturable increase in membrane conductance dependent on wavelength and light intensity in several different mammalian cell types including RBL, mast, HEK, PC12 and 3T3 cells. 3. The current was non-selective for cations and permeable to Ca2+, but was inhibited by trivalent cations and was not due to the activation of an endogenous ion channel. We termed this novel current I-LiNC for light-induced non-selective cation current. 4. A similar current was evoked by chemical peroxidants such as hydrogen peroxide and tertbutylhydroperoxide, but not by cytosolic oxidized glutathione. 5. The free-radical scavengers tocopherol (vitamin E) and ascorbic acid (vitamin C) significantly reduced the UV light effect. 6. The generation of the current was membrane delimited since it could be induced by the same UVA treatment in cell-free membrane patches showing a similar wavelength dependence. 7. These results suggest that I-LiNC is activated by UVA light-induced generation of free radicals acting through lipid or protein peroxidation, and may represent a ubiquitous mechanism by which Na+ and Ca2+ can enter cells after phototoxic or free radical-induced membrane damage.
引用
收藏
页码:365 / 377
页数:13
相关论文
共 22 条
[1]   NONNUCLEAR DAMAGE AND CELL-LYSIS ARE INDUCED BY UVA, BUT NOT UVB OR UVC, RADIATION IN 3 STRAINS OF L5178Y CELLS [J].
BEER, JZ ;
OLVEY, KM ;
MILLER, SA ;
THOMAS, DP ;
GODAR, DE .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1993, 58 (05) :676-681
[2]  
BOSE B, 1990, BIOTECHNOL APPL BIOC, V12, P557
[3]   LIPID-PEROXIDATION AND LOSS OF POTASSIUM FROM RED-BLOOD-CELLS PRODUCED BY PHOTOTOXIC QUINOLONES [J].
CARDENAS, AM ;
CORTES, MP ;
FERNANDEZ, E ;
PENA, W .
TOXICOLOGY, 1992, 72 (02) :145-151
[4]   THE MAMMALIAN ULTRAVIOLET RESPONSE IS TRIGGERED BY ACTIVATION OF SRC TYROSINE KINASES [J].
DEVARY, Y ;
GOTTLIEB, RA ;
SMEAL, T ;
KARIN, M .
CELL, 1992, 71 (07) :1081-1091
[5]   CA2+ AND MN2+ INFLUX THROUGH RECEPTOR-MEDIATED ACTIVATION OF NONSPECIFIC CATION CHANNELS IN MAST-CELLS [J].
FASOLATO, C ;
HOTH, M ;
MATTHEWS, G ;
PENNER, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :3068-3072
[6]  
FASOLATO C, 1993, J BIOL CHEM, V268, P20737
[7]  
Gies HP, 1995, PHOTOCHEM PHOTOBIOL, V62, P1015
[8]   SPECTRAL DEPENDENCE OF UV-INDUCED IMMEDIATE AND DELAYED APOPTOSIS - THE ROLE OF MEMBRANE AND DNA-DAMAGE [J].
GODAR, DE ;
LUCAS, AD .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1995, 62 (01) :108-113
[9]  
GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
[10]   Activation of Raf by ionizing radiation [J].
Kasid, U ;
Suy, S ;
Dent, P ;
Ray, S ;
Whiteside, TL ;
Sturgill, TW .
NATURE, 1996, 382 (6594) :813-816