Niveitalea solisilvae gen. nov., sp nov., isolated from forest soil and emended description of the genus Flavihumibacter Zhang et al. 2010

A Gram-stain-negative and strictly aerobic bacterial strain, designated 6-4(T), was isolated from forest soil in Jeju island, South Korea. Cells showing oxidase-positive and catalase-negative reactions were thin and long non-motile rods. Growth of strain 6-4(T) was observed at 20-35 degrees C (optimum, 30 degrees C) and pH 6.0-9.0 (optimum, pH 7.0). Strain 6-4(T) contained iso-C-15:0, anteiso-C-15:0, iso-C-15:1 G and iso-C-17:0 3-OH as the major fatty acids and menaquinone-7 (MK-7) as the sole isoprenoid quinone. Phosphatidylethanolamine was the major polar lipid and five unidentified aminolipids, one unidentified aminophospholipid and one unidentified lipid were also detected as minor polar lipids. The G+C content of the genomic DNA was 45.8 mol%. Strain 6-4(T) was most closely related to Flavihumibacter solisilvae 3-3(T) with a low 16S rRNA gene sequence similarity (94.2 %) and phylogenetic analysis indicated that the strain formed a distinct phylogenetic lineage from members of the genus Flavihumibacter and other closely related genera. On the basis of phylogenetic inference and phenotypic, chemotaxonomic and molecular properties, strain 6-4(T) represents a novel species of a new genus of the family Chitinophagaceae, for which the name Niveitalea solisilvae gen. nov., sp. nov. is proposed. The type strain of Niveitalea solisilvae is 6-4(T) (=KACC 18808(T)=JCM 31525(T)). An emended description of the genus Flavihumibacter is also proposed.