A novel antibody against the furin cleavage site of SARS-CoV-2 spike protein: Effects on proteolytic cleavage and ACE2 binding

被引：4

作者：

Spelios, Michael G. ^{[1
]}

Capanelli, Jeanne M. ^{[1
]}

Li, Adam W. ^{[1
]}

机构：

[1] EpiGentek Grp Inc, 110 Bi Cty Blvd,Suite 122, Farmingdale, NY 11735 USA

来源：

IMMUNOLOGY LETTERS | 2022年 / 242卷

关键词：

SARS-CoV-2; COVID-19; Spike; Furin; Proprotein convertase; ACE2; Furin site blocking antibody; CORONAVIRUS;

D O I：

10.1016/j.imlet.2022.01.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

SARS-CoV-2 harbors a unique S1/S2 furin cleavage site within its spike protein, which can be cleaved by furin and other proprotein convertases. Proteolytic activation of SARS-CoV-2 spike protein at the S1/S2 boundary facilitates interaction with host ACE2 receptor for cell entry. To address this, high titer antibody was generated against the SARS-CoV-2-specific furin motif. Using a series of innovative ELISA-based assays, this furin site blocking antibody displayed high sensitivity and specificity for the S1/S2 furin cleavage site, including with a P681R mutation, and demonstrated effective blockage of both enzyme-mediated cleavage and spike-ACE2 interaction. The results suggest that immunological blocking of the furin cleavage site may afford a suitable approach to stem proteolytic activation of SARS-CoV-2 spike protein and curtail viral infectivity.

引用

页码：1 / 7

页数：7