Direct STD NMR Identification of β-Galactosidase Inhibitors from a Virtual Dynamic Hemithioacetal System

被引：89

作者：

Caraballo, Remi ^{[1
]}

Dong, Hai ^{[1
]}

Ribeiro, Joao P. ^{[2
]}

Jimenez-Barbero, Jesus ^{[2
]}

Ramstrom, Olof ^{[1
]}

机构：

[1] Royal Inst Technol, KTH, Dept Chem, S-10044 Stockholm, Sweden

[2] Ctr Invest Biol, Madrid 28040, Spain

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 03期

基金：

瑞典研究理事会;

关键词：

beta-galactosidase; dynamic chemistry; hemithioacetals; inhibition; NMR spectroscopy; COMBINATORIAL LIBRARIES; LIGAND-BINDING; LIVING CELLS; ACTIVE-SITE; CHEMISTRY; ALDEHYDES; DECOMPOSITION; AMPLIFICATION; DISULFIDES; RESOLUTION;

D O I：

10.1002/anie.200903920

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) The formation of a dynamic hemithioacetal system and its application toward the discovery of ß-galactosidase inhibitors were successfully investigated. The designed dynamic system, which has a virtual character in neutral aqueous media, was subjected to a direct in situ identification of the best inhibitors by 1H STD NMR spectroscopy (ONPC : o-nitrophenyl-β-galactopyranoside). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：589 / 593

页数：5

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