Preparation of Silver-Chitosan Nanocomposites and Coating on Bandage for Antibacterial Wound Dressing Application

Bandage is a medical device that is essential for wound dressing. To improve the performance of the bandage, it has been coated by silver-chitosan nanocomposites (Ag/Chit) with pad-dry-cure method. The nanocomposites were performed by chemical reduction method at room temperature using glucose as reducing agent, sodium hydroxide (NaOH) as accelerator reagent, silver nitrate (AgNO3) as metal precursor and chitosan as stabilizing agent. Localized surface plasmon resonance (LSPR) absorption band of silver nanoparticles was investigated using UV-Vis spectrophotometer. The bandage coated Ag/Chit nanocomposites (B-Ag/Chit) were characterized by fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscope (SEM). In addition, antibacterial activity of the bandage toward Gram positive (Staphylococcus aureus) and Gram negative (Escherichia coli) were also studied. The formation of silver nanoparticles was confirmed by the appearance of LSPR absorption peak at 412.2 - 23.2 nm. Coating of nanocomposite cause increasing rigidity of bandage and decreasing on crystallinity. The bandages of B-Ag/Chit demonstrated good activity against both Gram positive (S. aureus) and Gram negative (E. Coli). Thus the bandages have a potential to be used for antibacterial wound dressing application.