Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation

被引:26
|
作者
Norrild, Rasmus K. [1 ]
Vettore, Nicola [1 ]
Coden, Alberto [1 ]
Xue, Wei-Feng [2 ]
Buell, Alexander K. [1 ]
机构
[1] Tech Univ Denmark, Dept Biotechnol & Biomed, Lyngby, Denmark
[2] Univ Kent, Sch Biosci, Canterbury, Kent, England
基金
英国生物技术与生命科学研究理事会;
关键词
Amyloid; Kinetics; Thermodynamics; Non-equilibrium system; Biosensing; Differential scanning fluorimetry; Quartz crystal microbalance;
D O I
10.1016/j.bpc.2021.106549
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. We use quartz crystal microbalance (QCM) and develop novel temperature perturbation experiments based on differential scanning fluorimetry (DSF) to measure the temperature dependence of the fibril growth and dissociation rates, allowing us to quantitatively describe the thermodynamic stability of PI3K-SH3 amyloid fibrils between 10 and 75 degrees C.
引用
收藏
页数:11
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