Biotransformation of hydroxycoumarins by the cultured cells of Nicotiana tabacum

被引：7

作者：

Hirata, T

Shimoda, K

Fujino, T

Ohta, S

机构：

[1] Hiroshima Univ, Grad Sch Sci, Dept Math & Life Sci, Higashihiroshima 7398526, Japan

[2] Hiroshima Univ, Instrument Ctr Chem Anal, Higashihiroshima 724, Japan

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2000年 / 10卷 / 05期

关键词：

Nicotiana tabacum; cultured cells; glucosylation; detoxification; hydroxycoumarin; scopoletin; DNA fragmentation; apoptosis-like cell death;

D O I：

10.1016/S1381-1177(00)00007-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Administration of hydroxycoumarins to the cultured cells of Nicotiana tabacum caused the ladder fragmentation of genomic DNA of the cells and the secretion of the intracellular phytoalexin, scopoletin, into the culture medium, and also the exogenous hydroxycoumarins were transformed into their corresponding beta-D-glucosides. The glucosylation seems to be a defense reaction of the cells for detoxification of toxic hydroxycoumarins, because their glucosides did not cause the DNA fragmentation. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：477 / 481

页数：5

共 17 条

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