Phenotypic and genetic characterization of Pseudomonas aeruginosa isolate COP2 from the lungs of COPD patients in China

被引：7

作者：

Zhao, Kelei ^{[1
]}

Yuan, Yang ^{[1
]}

Li, Jing ^{[1
]}

Pan, Wenjuan ^{[1
]}

Yan, Chaochao ^{[2
]}

Fu, Huiyao ^{[1
]}

Lin, Jiafu ^{[1
]}

Yue, Bisong ^{[2
]}

Wang, Xinrong ^{[1
]}

Gou, Xiaojun ^{[1
]}

Chu, Yiwen ^{[1
]}

Zhou, Yingshun ^{[3
]}

机构：

[1] Chengdu Univ, Sichuan Ind Inst Antibiot, Antibiot Res & Reevaluat Key Lab Sichuan Prov, Chengdu 610052, Sichuan, Peoples R China

[2] Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Bioresources & Ecoenvironm, Chengdu 610064, Sichuan, Peoples R China

[3] Southwest Med Univ, Dept Pathogen Biol, Coll Preclin Med, Luzhou 646000, Sichuan, Peoples R China

来源：

PATHOGENS AND DISEASE | 2019年 / 77卷 / 04期

基金：

中国国家自然科学基金;

关键词：

chronic obstructive pulmonary disease; Pseudomonas aeruginosa; phenotypic difference; phylogenetic status; transcriptional pattern; ACUTE EXACERBATION; READ ALIGNMENT; GENOME; IDENTIFICATION; ADAPTATION; VIRULENCE; BACTERIA; STRAINS; AIRWAYS; SIGNAL;

D O I：

10.1093/femspd/ftz038

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pseudomonas aeruginosa is an important opportunistic pathogen normally associated with increasing morbidity and mortality of immunocompromised hosts with respiratory infections. The phenotypic and genetic features of P. aeruginosa from patients with chronic obstructive pulmonary disease (COPD) remain poorly understood. By using the sputum samples of 25 hospitalized COPD patients from the affiliated hospital of Southwest Medical University (China), we identified a P. aeruginosa isolate, COP2, which showed multiple antibiotic resistance and enhanced Pseudomonas quinolone signal (PQS) production but decreased motility, biofilm formation and virulence compared with the model strain PAO1. Importantly, COP2 harbored a substantial amount of mutations that might influence the functions of 1771 genes in the genome and the evolutionary status of this isolate was clearly distinct from the PAO1 lineage. Accordingly, COP2 had a discrepant transcriptional pattern relating to flagellar assembly, antibiotic resistance, biofilm and PQS production, and can increase the capacities of compound degradation in response to resource/space stresses. Therefore, the identification of COP2 in this study provides preliminary information regarding the genetic features and survival strategy of P. aeruginosa in colonizing COPD lungs and lays the foundations for further understanding of the pathogenic mechanisms of pseudomonal infections.

引用

页数：9