Comparative evaluation of 16S rRNA gene in world-wide strains of Streptococcus iniae and Streptococcus parauberis for early diagnostic marker

被引：10

作者：

Mishra, Anshuman ^{[1
]}

Nam, Gyu-Hwi ^{[1
,2
]}

Gim, Jeong-An ^{[1
,2
]}

Seong, Minji ^{[3
]}

Choe, Yunjeong ^{[3
]}

Lee, Hee-Eun ^{[1
,2
]}

Jo, Ara ^{[1
,2
]}

Kim, Suhkmann ^{[4
]}

Kim, Do-Hyung ^{[5
]}

Cha, Hee-Jae ^{[6
,7
]}

Kang, Ho Young ^{[3
]}

Choi, Yung Hyun ^{[8
]}

Kim, Heui-Soo ^{[1
,2
]}

机构：

[1] Pusan Natl Univ, Genet Engn Inst, Busan 46241, South Korea

[2] Pusan Natl Univ, Coll Nat Sci, Dept Biol Sci, Busan 46241, South Korea

[3] Pusan Natl Univ, Coll Nat Sci, Dept Microbiol, Busan 46241, South Korea

[4] Pusan Natl Univ, Coll Nat Sci, Dept Chem, Busan 46241, South Korea

[5] Pukyong Natl Univ, Dept Aquat Life Med, Busan 48513, South Korea

[6] Kosin Univ, Coll Med, Dept Parasitol, Busan 49267, South Korea

[7] Kosin Univ, Coll Med, Dept Genet, Busan 49267, South Korea

[8] Dong Eui Univ, Coll Korean Med, Dept Biochem, Busan 47227, South Korea

来源：

GENES & GENOMICS | 2017年 / 39卷 / 07期

关键词：

Phylogenetic tree; Geography; Sequencing; Streptococcus iniae; Streptococcus parauberis; BACTERIAL IDENTIFICATION; SEQUENCE-ANALYSIS; SP-NOV; FISH; INFECTION; PATHOGEN; MICROORGANISMS; DATABASE; TILAPIA;

D O I：

10.1007/s13258-017-0542-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two bacterial etiological agents of the disease, Streptococcus iniae and Streptococcus parauberis has been associated with fish mortalities and heavy economic loss in all over the world. Bacterial identification based on 16S rRNA sequencing is very fast, accurate and reliable in comparison to other traditional phenotype methodologies. In this study, we investigate the usefulness of this method for diagnosis and identification of Streptococcus species. We have selected 61 phylogeographic strains of Streptococcus (34 strains of S. iniae and 27 strains of S. parauberis) and designed the universal primer against the identified most hypervariable region of the 16S rRNA gene. Our universal primer able to identify any geographical strains and offers a useful and fast alternative in a clinical laboratory under routine conditions. Based on our studies, we have developed an algorithm for appropriate control of S. iniae and S. parauberis disease. We suggested the phenotype observation along with universal primer combination to detect any kind of infection or carriers at early stages.

引用

页码：779 / 791

页数：13

共 58 条

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