Single-Molecule Redox Blinking of Perylene Diimide Derivatives in Water

被引：46

作者：

Cordes, Thorben ^{[1
]}

Vogelsang, Jan ^{[1
]}

Anaya, Milena ^{[2
]}

Spagnuolo, Carla ^{[2
]}

Gietl, Andreas ^{[1
]}

Summerer, Wolfram ^{[1
]}

Herrmann, Andreas ^{[3
]}

Muellen, Klaus ^{[2
]}

Tinnefeld, Philip ^{[1
]}

机构：

[1] Univ Munich, CeNS, D-80799 Munich, Germany

[2] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

[3] Univ Groningen, Dept Polymer Chem, Zernike Inst Adv Mat, NL-9747 AG Groningen, Netherlands

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 07期

关键词：

FLUORESCENCE SPECTROSCOPY; PHOTOPHYSICAL DYNAMICS; MICROSCOPY; DYES; DENDRIMER; NANOSCOPY; PATHWAYS;

D O I：

10.1021/ja9099714

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Dynamic developments in ultrasensitive and superresolution fluorescence microscopy call for improved fluorescence markers with increased photostability and new functionalities We used single-molecule spectroscopy to study water-soluble perylene dicarboximide fluorophores (PDI), which were immobilized in aqueous buffer by attaching the fluorophore to DNA Under these conditions bright fluorescence, comparable to that of single-molecule compatible organic fluorophores, is observed with homogeneous spectral and fluorescence decay time distributions. We additionally show how the fluorescence of the PDI can be controlled through photoinduced electron-transfer reactions by using different concentrations of reductants and oxidants, yielding either blinking or stable emission. We explain these properties by the redox potentials of PDI and the recently introduced ROXS (reducing and oxidizing system) concept Finally, we evaluate how this fluorescence control of PDIs can be used for superresolution "Blink-Microscopy" in aqueous or organic media and more generally for single-molecule spectroscopy

引用

页码：2404 / 2409

页数：6

共 43 条

[1] OPTICAL MODIFICATION OF A SINGLE IMPURITY MOLECULE IN A SOLID [J].