Biomimetic electrospun scaffolds from main extracellular matrix components for skin tissue engineering application - The role of chondroitin sulfate and sulfated hyaluronan

被引：62

作者：

Bhowmick, Sirsendu ^{[1
]}

Rother, Sandra ^{[1
]}

Zimmermann, Heike ^{[1
]}

Lee, Poh S. ^{[1
]}

Moeller, Stephanie ^{[2
]}

Schnabelrauch, Matthias ^{[2
]}

Koul, Veena ^{[3
]}

Jordan, Rainer ^{[4
]}

Hintze, Vera ^{[1
]}

Scharnweber, Dieter ^{[1
]}

机构：

[1] Tech Univ Dresden, Max Bergmann Ctr Biomat, Budapester Str 27, D-01069 Dresden, Germany

[2] INNOVENT eV, Biomat Dept, Prussingstr 27B, D-07745 Jena, Germany

[3] Indian Inst Technol Delhi, Ctr Biomed Engn, New Delhi 110016, India

[4] Tech Univ Dresden, Chair Macromol Chem, Dept Chem & Food Chem, Sch Sci, Mommsenstr 4, D-01069 Dresden, Germany

来源：

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2017年 / 79卷

关键词：

Sulfated glycosaminoglycans; Electrospun nanofibrous scaffolds; Cellular adhesion and proliferation; COLLAGEN I; GELATIN; GLYCOSAMINOGLYCANS; DIFFERENTIATION; RECOGNITION; MECHANISMS; RELEASE; CELLS; HMSC;

D O I：

10.1016/j.msec.2017.05.005

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Incorporation of bioactive components like glycosaminoglycans (GAGS) into "tissue engineering scaffolds, is a promising approach towards developing new generation functional biomaterial. Here, we have designed electrospun nanofibrous scaffolds made of gelatin and different concentrations of chemically sulfated or non-sulfated hyaluronan (sHA or HA) and chondroitin sulfate (CS). Evenly distributed fiber morphology was observed with no differences between varying concentrations and types of GAGs. In vitro release kinetics revealed that GAGs release is driven by diffusion. The effects of these scaffolds were analyzed on human keratinocyte (HaCaT), fibroblast (Hs27) and mesenchymal stem cells (hMSCs) adhesion and proliferation. A significant increase in cell number (similar to 5 fold) was observed when cultivating all three cell types alone on scaffolds containing sHA and CS. These findings suggest that sulfated GAG-containing electrospun nanofibrous scaffolds might be beneficial for the development of effective skin tissue engineered constructs by stimulating cellular performance and therefore accelerate epidermal-dermal regeneration processes. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：15 / 22

页数：8

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