On-line identification of 4"-isovalerylspiramycin I in the genetic engineered strain of S. spiramyceticus F21 by liquid chromatography with electrospray ionization tandem mass spectrometry, ultraviolet absorbance detection and nuclear magnetic resonance spectrometry

被引：4

作者：

Li, Jingyan ^{[1
,2
]}

Ma, Chunyan ^{[1
,2
]}

Wang, Hongyuan ^{[1
,2
]}

Wang, Yinghong ^{[2
,3
]}

Wu, Linzhuan ^{[1
,2
]}

Wang, Yiguang ^{[1
,2
]}

机构：

[1] Peking Union Med Coll, Minist Hlth, Key Lab Biotechnol Antibiot, Beijing 100050, Peoples R China

[2] Chinese Acad Med Sci, Beijing 100050, Peoples R China

[3] Peking Union Med Coll, Inst Mat Med, Minist Educ, Key Lab Bioact Subst & Resources Utilizat Chinese, Beijing 100050, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 08期

关键词：

Isovalerylspiramycin I; Spiramycin-like macrolides; Crude extract; Structural characterization; LC-DAD-UV-ESI-MSn; Stop-flow LC-H-1 NMR; LC-NMR; METABOLITES; COMPONENTS; LC/MS; MS;

D O I：

10.1016/j.chroma.2009.12.072

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

LC-hyphenated techniques were applied to the on-line identification of isovalerylspiramycin I (isp I), a spiramycin-like macrolide in the crude extract of fermentation broth from a genetically engineered strain of S. spiramyceticus F21. In the structural characterization of the large molecular secondary metabolite of isp I, LC-DAD-UV-ESI-MSn analysis played a crucial sole, and stop-flow LC-H-1 NMR measurement, with bitespiramycin used as reference, was a Valuable complement approach This rational approach proved to be ail efficient means for the rapid anti accurate structural determination of known microbial secondary metabolites, by which targeted isolation of component(s) of interest can he subsequently pet formed for further biological and pharmacological studies in drug development (C) 2009 Elsevier B.V All I fights reserved

引用

页码：1419 / 1424

页数：6

共 25 条

[1] ONLINE USE OF NMR DETECTION IN SEPARATION CHEMISTRY [J].