Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis

被引：44
作者：
Lagaert, Stijn
Van Campenhout, Steven
Pollet, Annick
Bourgois, Tine M.
Delcour, Jan A.
Courtin, Christophe M.
Volckaert, Guido
机构：
[1] Katholieke Univ Leuven, Div Gene Technol, B-3001 Louvain, Belgium
[2] Katholieke Univ Leuven, Lab Food Chem & Biochem, B-3001 Louvain, Belgium
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 16期
关键词：
D O I：
10.1128/AEM.00722-07
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The family 8 glycoside hydrolase (RexA) from Bifidobacterium adolescentis was expressed in Escherichia coli. The recombinant enzyme was characterized as a reducing-end xylose-releasing exo-oligoxylanase. Apart from giving insights into this new class of enzymes, knowledge of the RexA enzyme helps to postulate a mechanism for the B. adolescentis breakdown of prebiotic xylooligosaccharides.
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页码：5374 / 5377
页数：4
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