Study on the Interaction between DNR-D3 (Daunorubicin Derivative) and ctDNA by Spectroscopic Methods

被引：0
|
作者：
Lue Juan [1 ]
Wang Gong-ke [1 ]
Zhang Gui-sheng [1 ]
Liu Qing-feng [1 ]
Lu Yan [1 ]
机构：
[1] Henan Normal Univ, Coll Chem & Environm Sci, Xinxiang 453007, Peoples R China
来源：
SPECTROSCOPY AND SPECTRAL ANALYSIS | 2010年 / 30卷 / 05期
关键词：
DNR-D3; ctDNA; UV spectrum; Fluorescence spectrum; Anticancer activity; BOVINE SERUM-ALBUMIN; BIOLOGICAL-ACTIVITIES; DNA-BINDING; THERMODYNAMICS; MITOXANTRONE; ANALOGS; DRUGS;
D O I：
10.3964/j.issn.1000-0593(2010)05-1324-05
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
The interaction of DNR-D3(daunorubicin derivative) synthesized in our laboratory with ctDNA was investigated by UV spectrum and fluorescence spectrum under physiological conditions (pH 7.4) for the first time. The red shifts and hypochromicities were observed from the absorption titration experiments. These results suggest that DNR-D3 was intercalated into the DNA base pairs. Through the fluorescence quenching data measured at different temperatures (20 degrees C, 30 degrees C and 37 degrees C), it is known that the quenching mechanism of fluorescence of DNR-D3 by ctDNA is a static quenching type. On the other hand, the binding constant, the number of binding sites and thermodynamic parameters were also obtained. These data also indicate that the binding mode of the interaction between DNR-D3 and ctDNA is intercalation. Additionally, the types of interaction force are mainly hydrogen bonding and electrostatic interaction, and the binding is exothermic enthalpy-entropy cooperative driven process. When the degree of fluorescence quenching of DNR-D3 is 50%, the ratio of the molar concentration of DNR-D3 to ctDNA is 7/25, which indicates that the DNR-D3 anthracycline was intercalated into the DNA base pairs and that DNR-D3 showed a strong anticancer activity. DNR-D3 is expected to become one of the drug candidates from our investigations.
引用
收藏
页码：1324 / 1328
页数：5
相关论文
共 19 条
  • [1] Investigation of the interaction between trazodone hydrochloride and bovine serum albumin
    Ashoka, S.
    Seetharamappa, J.
    Kandagal, P. B.
    Shaikh, S. M. T.
    [J]. JOURNAL OF LUMINESCENCE, 2006, 121 (01) : 179 - 186
  • [2] DNA-binding of drugs used in medicinal therapies
    Bischoff, G
    Hoffmann, S
    [J]. CURRENT MEDICINAL CHEMISTRY, 2002, 9 (03) : 321 - 348
  • [3] Comparison of DNA sequence selectivity of anthracycline antibiotics and their 3'-hydroxylated analogs
    Jolles, B
    Laigle, A
    Priebe, W
    GarnierSuillerot, A
    [J]. CHEMICO-BIOLOGICAL INTERACTIONS, 1996, 100 (02) : 165 - 176
  • [4] INTERACTIONS OF ANTITUMOR AGENTS AMETANTRONE AND MITOXANTRONE (NOVATRONE) WITH DOUBLE-STRANDED DNA
    KAPUSCINSKI, J
    DARZYNKIEWICZ, Z
    [J]. BIOCHEMICAL PHARMACOLOGY, 1985, 34 (24) : 4203 - 4213
  • [5] THE BINDING OF ORGANIC IONS BY PROTEINS - EFFECT OF TEMPERATURE
    KLOTZ, IM
    URQUHART, JM
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1949, 71 (03) : 847 - 852
  • [6] DNA-BINDING STUDIES AND SITE-SELECTIVE FLUORESCENCE SENSITIZATION OF AN ANTHRYL PROBE
    KUMAR, CV
    ASUNCION, EH
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (19) : 8547 - 8553
  • [7] Interaction of anticancer drug mitoxantrone with DNA analyzed by electrochemical and spectroscopic methods
    Li, N
    Ma, Y
    Yang, C
    Guo, LP
    Yang, XR
    [J]. BIOPHYSICAL CHEMISTRY, 2005, 116 (03) : 199 - 205
  • [8] Study on the interaction between DNA and protein induced by anticancer drug carboplatin
    Liu, SC
    Liu, YJ
    Li, J
    Guo, ML
    Nie, LH
    Yao, SZ
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2005, 63 (02): : 125 - 136
  • [9] Liu XF, 2004, ACTA CHIM SINICA, V62, P1484
  • [10] THERMODYNAMICS OF PROTEIN ASSOCIATION REACTIONS - FORCES CONTRIBUTING TO STABILITY
    ROSS, PD
    SUBRAMANIAN, S
    [J]. BIOCHEMISTRY, 1981, 20 (11) : 3096 - 3102
12