First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

被引：24

作者：

He, Lan ^{[1
,2
]}

Miao, Xiaoyan ^{[1
,2
]}

Hu, Jinfang ^{[1
,2
]}

Huang, Yuan ^{[1
,2
]}

He, Pei ^{[1
,2
]}

He, Junwei ^{[1
,2
]}

Yu, Long ^{[1
,2
]}

Malobi, Ngabu ^{[1
,2
]}

Shi, Ligang ^{[3
]}

Zhao, Junlong ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China

[2] Huazhong Agr Univ, Minist Agr, Key Lab Dev Vet Diagnost Prod, Wuhan, Hubei, Peoples R China

[3] Luoyang Ctr Anim Dis Control & Prevent, Luoyang, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2017年 / 8卷

基金：

中国国家自然科学基金;

关键词：

Babesia gibsoni; babesiosis; reverse line blot; 18S rRNA; pet dog; companion animal; RIBOSOMAL-RNA GENE; THEILERIA-PARVA; INFECTION; IDENTIFICATION; CATTLE; MORTALITY; VARIANTS; AREAS; BLOOD; TICKS;

D O I：

10.3389/fmicb.2017.01577

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Canine piroplasmosis is a significant disease in dogs caused by Babesia and Theileria parasites. The clinical manifestations range from mild illness to serious disease depending on the parasite species and the physical condition of the infected dog. Canine piroplasmosis has been reported to be prevalent in China. However, no molecular evidence of the disease has been reported in pet dogs from Wuhan. In this study, 118 blood samples were randomly collected from pet dogs in veterinary clinics. The blood samples were subjected to both microscopic examination and reverse line blot (RLB) hybridization assays to detect piroplasm infection. Parasites were observed in 10 blood samples via microscopic examination, whereas there were 14 Babesia gibsoni-positive RLB tests. Phylogenetic analysis was performed after the 18S rRNA and ITS gene sequences from the 14 positive samples were cloned and sequenced. The results confirmed the existence of B. gibsoni in this area. This is the first molecular report of canine babesiosis in pet dogs from Wuhan, China. Pet dogs are companion animals, and the prevalence of babesiosis will be of concern in daily life. This study will help veterinarians better understand the prevalence of canine babesiosis and provide a guide for disease control in pet dogs.

引用

页数：6

共 41 条

[1] The risk of vector-borne infections in sled dogs associated with existing and new endemic areas in Poland. Part 2: Occurrence and control of babesiosis in a sled dog kennel during a 13-year-long period [J].