Usutu virus in wild birds in northern Italy

被引：84

作者：

Manarolla, G. ^{[1
]}

Bakonyi, T. ^{[2
,3
]}

Gallazzi, D. ^{[1
]}

Crosta, L. ^{[4
]}

Weissenboeck, H. ^{[5
]}

Dorrestein, G. M. ^{[6
]}

Nowotny, N. ^{[2
]}

机构：

[1] Univ Milan, Dipartimento Patol Anim Igiene & Sanita Pubbl Vet, I-20133 Milan, Italy

[2] Univ Vet Med Vienna, Dept Pathobiol, Zoonoses & Emerging Infect Grp, A-1210 Vienna, Austria

[3] Szent Istvan Univ, Dept Microbiol & Infect Dis, Fac Vet Sci, H-1143 Budapest, Hungary

[4] Ctr Serv Vet Valcurone SRL, I-23873 Missaglia, LC, Italy

[5] Univ Vet Med Vienna, Dept Pathobiol, Inst Pathol & Forens Vet Med, A-1210 Vienna, Austria

[6] NOIVBD, Diagnost Pathol Lab, NL-5507 PP Veldhoven, Netherlands

来源：

VETERINARY MICROBIOLOGY | 2010年 / 141卷 / 1-2期

关键词：

Usutu virus; Wild birds; Immunohistochemistry; RT-PCR; Sequencing; INFECTIONS; EMERGENCE; AUSTRIA;

D O I：

10.1016/j.vetmic.2009.07.036

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Usutu virus (USUV) infection was diagnosed in two free-living blackbirds and in three captive owls belonging to two different species in northern Italy in the summers of 2006-2008. Diagnosis was established by immunohistochemistry and RT-PCR. RT-PCR was performed on frozen and on paraffin-embedded tissues (PET), respectively. From the frozen samples a partial sequence of the putative USUV E and NS1 proteins (1229 bp) was determined, whereas partial sequences of the putative NS3 (278 bp) and NS5 (159 bp) proteins were obtained from PET. Additionally, one partial sequence (163 bp) of the putative 3'UTR region was determined from all samples. Sequencing of the amplification products revealed 99.8-100% nucleotide identity of the Italian USUV strains to those from other central European countries. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：159 / 163

页数：5

共 13 条

[1] Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001 -: Comparison with the South African strain SAAR-1776 and other flaviviruses
Bakonyi, T
Gould, EA
Kolodziejek, J
Weissenböck, H
Nowotny, N
[J]. VIROLOGY, 2004, 328 (02) : 301 - 310
[2] Emergence of Usutu virus in Hungary
Bakonyi, Tamas
Erdelyi, Karoly
Ursu, Krisztina
Ferenczi, Emoke
Csoergo, Tibor
Lussy, Helga
Chvala, Sonja
Bukovsky, Christiane
Meister, Tanja
Weissenboeck, Herbert
Nowotny, Norbert
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (12) : 3870 - 3874
[3] Monitoring of Usutu virus activity and spread by using dead bird surveillance in Austria, 2003-2005
Chvala, S.
Bakonyi, T.
Bukovsky, C.
Meister, T.
Brugger, K.
Rubel, F.
Nowotny, N.
Weissenboeck, H.
[J]. VETERINARY MICROBIOLOGY, 2007, 122 (3-4) : 237 - 245
[4] Pathology and viral distribution in fatal usutu virus infections of birds from the 2001 and 2002 outbreaks in Austria
Chvala, S
Kolodziejek, J
Nowotny, N
Weissenböck, H
[J]. JOURNAL OF COMPARATIVE PATHOLOGY, 2004, 131 (2-3) : 176 - 185
[5] Cole R.A., 1999, Miscellaneous parasitic diseases in Field manual of wildlife diseases: general field procedures and diseases of birds, P249
[6] Phylogeny of the genus Flavivirus
Kuno, G
Chang, GJJ
Tsuchiya, KR
Karabatsos, N
Cropp, CB
[J]. JOURNAL OF VIROLOGY, 1998, 72 (01) : 73 - 83
[7] Serological evidence of USUTU virus occurrence in north-eastern Italy
Lelli, R.
Savini, G.
Teodori, L.
Filipponi, G.
Di Gennaro, A.
Leone, A.
Di Gialleonardo, L.
Venturi, L.
Caporale, V.
[J]. ZOONOSES AND PUBLIC HEALTH, 2008, 55 (07) : 361 - 367
[8] West Nile virus circulation detected in northern Italy in sentinel chickens
Rizzoli, Annapaola
Rosa, Roberto
Rosso, Fausta
Buckley, Alan
Gould, Ernest
[J]. VECTOR-BORNE AND ZOONOTIC DISEASES, 2007, 7 (03) : 411 - 417
[9] Steinmetz H.W., 2007, Erkrankungen der Zootiere, V43, P129
[10] The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools
Thompson, JD
Gibson, TJ
Plewniak, F
Jeanmougin, F
Higgins, DG
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (24) : 4876 - 4882

← 1 2 →