The distribution and position of fatty acids in glycerides hydrolyzed from fish oil by lipase

被引:0
作者
Hur, BK [1 ]
Hu, HB
Yun, HS
机构
[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea
[2] Zhejiang Univ, Dept Chem & Biochem Engn, Hangzhou 310027, Peoples R China
关键词
fatty acid; DHA; EPA; hydrolysis; migration; 1.3-positional specificity;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In order to determine the position and the content of fatty acids attached to glycerides and the migration degree of fatty acids in the migration reaction, fish oil was hydrolyzed with lipolase-100T which was derived from Aspergillus oryzae. The content of fatty acids in the glyceride mixture was analyzed and compared with that of fish oil. The amounts of fatty acid in a 2-position and the migration degree of the fatty acid in 2,3-DG (diglyceride) and 2-MG (monoglyceride) were carefully calculated. The results showed that approximately 95% (w/w) of DHA (docosahexaenoic acid) and 65% of EPA (eicosapentaenoic acid) were attached to the 2-position of glycerides in fish oil. Approximately 87% (w/w) Of DHA and 75% of EPA remained in 2,3-DG, and 88% of DHA and 65% of EPA in 2-MG were not involved in the migration reaction.
引用
收藏
页码:502 / 506
页数:5
相关论文
共 14 条
[1]  
[Anonymous], 1986, ANAL OILS FATS
[2]   BIOLOGICAL EFFECTS OF FISH OILS IN RELATION TO CHRONIC DISEASES [J].
CARROLL, KK .
LIPIDS, 1986, 21 (12) :731-732
[3]   THE PREPARATION OF HOMOGENEOUS TRIGLYCERIDES OF EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID BY LIPASE [J].
HARALDSSON, GG ;
GUDMUNDSSON, BO ;
ALMARSSON, O .
TETRAHEDRON LETTERS, 1993, 34 (36) :5791-5794
[4]   ENZYMATIC PREPARATION OF MONOGLYCERIDES IN MICROEMULSION [J].
HOLMBERG, K ;
OSTERBERG, E .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1988, 65 (09) :1544-1548
[5]  
Hur BK, 1999, J MICROBIOL BIOTECHN, V9, P624
[6]  
Jung JY, 1997, J MICROBIOL BIOTECHN, V7, P151
[7]  
*KSTM, 1985, 2731 KSTM
[8]   HUMAN ABSORPTION OF FISH OIL FATTY-ACIDS AS TRIACYLGLYCEROLS, FREE ACIDS, OR ETHYL-ESTERS [J].
LAWSON, LD ;
HUGHES, BG .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 152 (01) :328-335
[9]  
LEPAGE G, 1984, J LIPID RES, V25, P1391
[10]   LIPASE-CATALYZED INTERESTERIFICATION OF OILS AND FATS [J].
MACRAE, AR .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1983, 60 (02) :291-294